Young M R, Newby M
Cancer Res. 1986 Jan;46(1):160-4.
The role of macrophages in tumor metastasis was examined by using migration of a cloned metastatic Lewis lung carcinoma (LLC) variant, LLC-C4, out of glass capillary tubes as an in vitro model for dissemination of tumor cells from a primary tumor mass. Macrophages, derived from LLC tumors of C57BL/6 mice or from peritoneal exudates of mice given injections of complete Freund's adjuvant, enhanced tumor cell migration through an indomethacin-sensitive mechanism. Resident peritoneal macrophages did not produce a tumor migration-enhancing activity but could be induced to do so by preincubation with LLC-C4 cells or their culture supernatants. The capacity of macrophages to enhance LLC-C4 migration corresponded to their secretion of prostaglandin E2. Addition of similar concentrations of prostaglandin E2 to the migration medium of LLC-C4 cells enhanced their migration out of the capillary tubes. These results suggest that macrophages, following exposure to tumor cells or their products or following stimulation with complete Freund's adjuvant, secrete elevated amounts of prostaglandin E2 which in turn may enhance tumor dissemination.
通过使用克隆的转移性Lewis肺癌（LLC）变体LLC-C4从玻璃毛细管中迁移作为肿瘤细胞从原发性肿瘤块中扩散的体外模型，研究了巨噬细胞在肿瘤转移中的作用。源自C57BL/6小鼠的LLC肿瘤或注射完全弗氏佐剂的小鼠腹膜渗出液的巨噬细胞，通过一种对吲哚美辛敏感的机制增强肿瘤细胞迁移。驻留腹膜巨噬细胞不产生肿瘤迁移增强活性，但通过与LLC-C4细胞或其培养上清液预孵育可诱导产生。巨噬细胞增强LLC-C4迁移的能力与其前列腺素E2的分泌相对应。向LLC-C4细胞的迁移培养基中添加相似浓度的前列腺素E2可增强其从毛细管中迁移出来的能力。这些结果表明，巨噬细胞在接触肿瘤细胞或其产物后或在用完全弗氏佐剂刺激后，分泌升高量的前列腺素E2，这反过来可能增强肿瘤扩散。
