Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
Irion animal hospital, Seoul, Korea.
Vet Ophthalmol. 2021 Sep;24(5):520-532. doi: 10.1111/vop.12937. Epub 2021 Sep 23.
To analyze proteomic profiles of the aqueous humor (AH) of canines with primary angle-closure glaucoma (PACG) and identify associated protein alterations.
Six American Cocker Spaniels with PACG and six American Cocker Spaniels without ocular diseases.
Aqueous humor samples were collected from six American Cocker Spaniels with PACG at Seoul National University, VMTH, and six healthy Cocker Spaniels without ocular disease at Irion Animal Hospital. For the PACG group, AH samples were obtained by anterior chamber paracentesis prior to glaucoma treatment. For the AH control group, AH samples were collected from patients anesthetized for other reasons. Total AH protein concentration was determined by the bicinchoninic acid (BCA) assay. AH protein samples were quantified by liquid chromatography-mass spectrometry (LC-MS/MS). Raw MS spectra were processed using MaxQuant software 30, and the Gene Ontology (GO) enrichment analysis was performed using ClueGO.
The AH protein concentration in the PACG group (10.49 ± 17.98 µg/µl) was significantly higher than that of the control group (0.45 ± 0.11 µg/µl; p < .05). A total of 758 proteins were identified in the AH. Several proteins both significantly increased (n = 69) and decreased (n = 252) in the PACG group compared to those in the control group. GO enrichment analysis showed that the "response to wounding," "negative regulation of endopeptidase activity," and "cell growth" pathways were the most enriched terms in the PACG group compared to the control group. The top 5 proteins that were significantly increased in the AH of the PACG group were secreted phosphoprotein 1 (SPP1), peptidoglycan recognition proteins 2 (PGLYRP2), tyrosine 3-monooxygenase (YWHAE), maltase-glucoamylase (MGAM), and vimentin (VIM).
Gene Ontology enrichment analysis using the proteomic data showed that proteins and pathways related to inflammation were significantly upregulated in the various stage of PACG. Proteomic analysis of the AH from the PACG may provide valuable insights into PACG pathogenesis.
分析原发性闭角型青光眼（PACG）犬的房水（AH）的蛋白质组谱，并鉴定相关的蛋白质变化。
6 只患有 PACG 的美国可卡犬和 6 只无眼部疾病的美国可卡犬。
在首尔国立大学 VMTH 收集 6 只患有 PACG 的美国可卡犬的房水样本，在 Irion 动物医院收集 6 只无眼部疾病的健康可卡犬的房水样本。对于 PACG 组，在青光眼治疗前通过前房穿刺术获得 AH 样本。对于 AH 对照组，从因其他原因麻醉的患者中收集 AH 样本。通过双缩脲法（BCA）测定总 AH 蛋白浓度。通过液相色谱-质谱联用（LC-MS/MS）定量 AH 蛋白样品。使用 MaxQuant 软件 30 处理原始 MS 谱图，并使用 ClueGO 进行基因本体论（GO）富集分析。
PACG 组（10.49 ± 17.98 µg/µl）的 AH 蛋白浓度明显高于对照组（0.45 ± 0.11 µg/µl；p <.05）。在 AH 中鉴定出 758 种蛋白质。与对照组相比，PACG 组中许多蛋白质明显增加（n = 69）和减少（n = 252）。GO 富集分析表明，与对照组相比，PACG 组中“对创伤的反应”、“内肽酶活性的负调节”和“细胞生长”途径是最富集的术语。在 PACG 组的 AH 中显著增加的前 5 种蛋白质是分泌磷蛋白 1（SPP1）、肽聚糖识别蛋白 2（PGLYRP2）、酪氨酸 3-单加氧酶（YWHAE）、麦芽糖-葡糖苷酶（MGAM）和波形蛋白（VIM）。
使用蛋白质组学数据进行的基因本体论富集分析表明，与炎症相关的蛋白质和途径在 PACG 的各个阶段均显著上调。PACG 的 AH 蛋白质组学分析可能为 PACG 的发病机制提供有价值的见解。
