Yonsei University College of Medicine, Seoul, Korea.
Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.
Yonsei Med J. 2021 Oct;62(10):936-942. doi: 10.3349/ymj.2021.62.10.936.
A critical indicator of the overall survival of patients with high-grade glioma is the successful isolation of tumor mesenchymal stem-like cells (tMSLCs), which play important roles in glioma progression. However, attempts to isolate tMSLCs from surgical specimens have not always been successful, and the reasons for this remain unclear. Considering that the amount of surgical high-grade glioma specimens varies, we hypothesized that larger surgical specimens would be better for tMSLC isolation.
We assessed 51 fresh, high-grade glioma specimens and divided them into two groups according to the success or failure of tMSLC isolation. The success of tMSLC isolation was confirmed by plastic adherence, presenting antigens, tri-lineage differentiation, and non-tumorigenicity. Differences in characteristics between the two groups were tested using independent two sample t-tests, chi-square tests, or Kaplan-Meier survival analysis.
The mean specimen weights of the groups differed from each other (tMSLC-negative group: 469.9±341.9 mg, tMSLC positive group: 546.7±618.9 mg), but the difference was not statistically significant. The optimal cut-off value of specimen weight was 180 mg, and the area under the curve value was 0.599.
Our results suggested a minimum criterion for specimen collection, and found that the specimen amount was not deeply related to tMSLC detection. Collectively, our findings imply that the ability to isolate tMSLCs is determined by factors other than the specimen amount.
高级别神经胶质瘤患者的总体存活率的一个关键指标是成功分离肿瘤间质干细胞样细胞（tMSLCs），其在神经胶质瘤的进展中发挥重要作用。然而，从手术标本中分离 tMSLCs 的尝试并不总是成功的，其原因尚不清楚。鉴于手术高级别神经胶质瘤标本的数量不同，我们假设更大的手术标本更有利于 tMSLC 分离。
我们评估了 51 例新鲜的高级别神经胶质瘤标本，并根据 tMSLC 分离的成功或失败将其分为两组。通过塑料附着、呈现抗原、三系分化和非致瘤性来确认 tMSLC 分离的成功。使用独立两样本 t 检验、卡方检验或 Kaplan-Meier 生存分析测试两组之间的特征差异。
两组标本的平均重量不同（tMSLC 阴性组：469.9±341.9mg，tMSLC 阳性组：546.7±618.9mg），但差异无统计学意义。标本重量的最佳截断值为 180mg，曲线下面积值为 0.599。
我们的结果表明了一个最小的标本采集标准，并发现标本量与 tMSLC 检测没有密切关系。总之，我们的研究结果表明，分离 tMSLCs 的能力取决于标本量以外的因素。
