College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, 163000, PR China.
Institute of Tropical Bioscience and Biotechnology, Hainan Academy of Tropical Agricultural Resource, CATAS, Haikou 571101, PR China.
Int J Syst Evol Microbiol. 2021 Sep;71(9). doi: 10.1099/ijsem.0.005024.
A Gram-stain-positive and motile bacterial strain, designated IB182363, was isolated from surface seawater of the South China Sea. Cells grew at pH 5.0-9.5 (optimum, pH 7.0-8.0), 20-40 °C (optimum, 30 °C) and with 1-8 % (w/v) NaCl (optimum, 2-4 %). On the basis of 16S rRNA gene sequence analysis, strain IB182363 was affiliated to the genus and the closest phylogenetically related species was DSM18677 with 96.9 % sequence similarity. The values of whole genome average nucleotide identity analysis and digital DNA-DNA hybridization between the isolate and the closely related type strains were less than 86.3 and 25.6 %, respectively. Chemotaxonomic analysis revealed that strain IB182363 possessed -diaminopimelic acid in the cell-wall peptidoglycan and contained menaquinone MK-7 as the predominant isoprenoid quinone. The major cellular fatty acids were anteiso-C, C and iso-C. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid, two unidentified aminolipids, two unidentified phospholipids and four unidentified aminophospholipids. The genomic DNA G+C content was 54.5 mol%. On the basis of the above results, strain IB182363 represents a novel species of the genus , for which we propose the name sp. nov. with the type strain IB182363 (=MCCC 1K04630=JCM 34214).
一株革兰氏阳性、运动的细菌菌株，被命名为 IB182363，从南海的表层海水中分离得到。细胞在 pH5.0-9.5（最佳 pH7.0-8.0）、20-40°C（最佳 30°C）和 1-8%（w/v）NaCl（最佳 2-4%）下生长。基于 16S rRNA 基因序列分析，菌株 IB182363 属于 属，与亲缘关系最近的种是 DSM18677，相似度为 96.9%。该分离株与亲缘关系最近的模式株之间的全基因组平均核苷酸同一性分析和数字 DNA-DNA 杂交值分别小于 86.3%和 25.6%。化学分类分析表明，菌株 IB182363 的细胞壁肽聚糖中含有二氨基庚二酸，并且含有 MK-7 作为主要的异戊烯醌。主要的细胞脂肪酸为 anteiso-C15:0、C16:0 和 iso-C15:0。极性脂包括磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油、一种未鉴定的糖脂、两种未鉴定的胺脂、两种未鉴定的磷脂和四种未鉴定的氨基磷脂。基因组 DNA G+C 含量为 54.5mol%。基于上述结果，菌株 IB182363 代表了 属的一个新种，我们建议将其命名为 sp. nov.，其模式菌株为 IB182363（=MCCC 1K04630=JCM 34214）。
