Paenibacillus translucens sp. nov., isolated from tidal flat sediment.

A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11, was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea. Strain CJ11 grew optimally on R2A at 30 °C and pH 7.0. Sequencing results of the 16S rRNA gene revealed that strain CJ11 possesses two copies of the 16S rRNA gene varying at five nucleotide positions. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11 belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691 (99.36-99.15 % similarity). DNA-DNA relatedness levels of strain CJ11 was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691. The G+C content of the genomic DNA was 51.0 mol%. Strain CJ11contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major isoprenoid quinone was menaquinone-7. The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids. On the basis of the polyphasic taxonomic study, strain CJ11 represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp. nov. is proposed. The type strain is CJ11 (=KACC 19304=JCM 32080).