镍氧化物纳米颗粒对多刺步甲（Forskl, 1775）（鞘翅目：步甲科）的生化和组织学影响。
Biochemical and histological alterations induced by nickel oxide nanoparticles in the ground beetle Blaps polychresta (Forskl, 1775) (Coleoptera: Tenebrionidae).
机构信息
College of Life Science and Engineering, Foshan University, Foshan, Guangdong, China.
Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh, Egypt.
出版信息
PLoS One. 2021 Sep 24;16(9):e0255623. doi: 10.1371/journal.pone.0255623. eCollection 2021.
The present study evaluates the effect of nickel oxide nanoparticles on some biochemical parameters and midgut tissues in the ground beetle Blaps polychresta as an indicator organism for nanotoxicity. Serial doses of the NiO-NPs colloid (0.01, 0.02, 0.03, 0.04, 0.05, and 0.06 mg/g) were prepared for injecting into the adult beetles. Insect survival was reported daily for 30 days, and the sublethal dose of 0.02 mg/g NiO-NPs was selected for the tested parameters. After the treatment, nickel was detected in the midgut tissues by X-ray microanalysis. The treated group demonstrated a significant increase in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities when compared to the untreated group. However, the treated group demonstrated a significant decrease in ascorbate peroxidase (APOX) activity when compared to the untreated group. Histological and ultrastructural changes in the midgut tissues of treated and untreated beetles were also observed. The current findings provide a precedent for describing the physiological and histological changes caused by NiO-NPs in the ground beetle B. polychresta.
本研究评估了氧化镍纳米颗粒对作为纳米毒性指示生物的步甲 Blaps polychresta 某些生化参数和中肠组织的影响。制备了一系列 NiO-NPs 胶体浓度（0.01、0.02、0.03、0.04、0.05 和 0.06mg/g），用于注入成虫甲虫体内。每天报告昆虫的存活率，为期 30 天，并选择亚致死剂量的 0.02mg/g NiO-NPs 进行测试参数。处理后，通过 X 射线微分析检测到中肠组织中的镍。与未处理组相比，处理组的天冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT）活性显著增加。然而，与未处理组相比，处理组的抗坏血酸过氧化物酶（APOX）活性显著降低。还观察到处理和未处理甲虫中肠组织的组织学和超微结构变化。目前的研究结果为描述 NiO-NPs 在步甲 B. polychresta 中引起的生理和组织学变化提供了先例。
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