Zhejiang Provincial Key Lab of Geriatrics & Geriatrics Institute of Zhejiang Province, Department of Geriatrics, Zhejiang Hospital, Hangzhou 310030, PR China.
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Carbohydr Polym. 2021 Dec 1;273:118510. doi: 10.1016/j.carbpol.2021.118510. Epub 2021 Jul 30.
Human cytomegalovirus (HCMV) remains a major public health burden worldwide. The anti-HCMV activity of glucuronomannan oligosaccharides (Gs) and sulphated glucuronomannan oligosaccharides (SGs) was investigated. Among these Gs and SGs, G4S1 and G6S1 (higher sulphated glucuronomannan tetramer and hexamer) showed satisfactory anti-HCMV activity starting at 50 μg/mL and 10 μg/mL, respectively. The results of the morphology, western blotting, qPCR and TCID assay showed that they prevented lytic cytopathic changes, inhibited the expression of IE1/2 and UL44, and reduced the UL123 copy number and virus titre significantly. It was interesting to note that degree of sulphation and polymerization was more important for anti-HCMV activity. Moreover, the anti-HCMV activities of G4S1 and G6S1 were stable when stored at 4 °C, -20 °C, and -80 °C for at least three months and mainly occurred in the early stage of HCMV infection through the negative charge of the sulphate groups and the interaction between SGs and the host cells.
人巨细胞病毒（HCMV）仍然是全球主要的公共卫生负担。研究了葡聚糖甘露聚糖寡糖（Gs）和硫酸葡聚糖甘露聚糖寡糖（SGs）的抗 HCMV 活性。在这些 Gs 和 SGs 中，G4S1 和 G6S1（更高硫酸化的葡聚糖甘露聚糖四聚体和六聚体）在 50μg/mL 和 10μg/mL 时表现出令人满意的抗 HCMV 活性。形态学、western blot、qPCR 和 TCID 测定结果表明，它们可预防裂解细胞病变，抑制 IE1/2 和 UL44 的表达，并显著降低 UL123 拷贝数和病毒滴度。有趣的是，硫酸化和聚合度对抗 HCMV 活性更为重要。此外，G4S1 和 G6S1 的抗 HCMV 活性在 4°C、-20°C 和-80°C 下储存至少三个月时是稳定的，主要通过硫酸基团的负电荷和 SGs 与宿主细胞之间的相互作用发生在 HCMV 感染的早期阶段。
