College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, China.
Department of Clinical Laboratory, Center for Gene Diagnosis, and Program of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China.
Nat Commun. 2021 Sep 24;12(1):5629. doi: 10.1038/s41467-021-25804-z.
Severe fever with thrombocytopenia syndrome (SFTS) is a novel tick-borne infectious disease caused by a new type of SFTS virus (SFTSV). Here, a longitudinal sampling study is conducted to explore the differences in transcript levels after SFTSV infection, and to characterize the transcriptomic and epigenetic profiles of hospitalized patients. The results reveal significant changes in the mRNA expression of certain genes from onset to recovery. Moreover, mA-seq reveals that certain genes related with immune regulation may be regulated by mA. Besides the routine tests such as platelet counts, serum ALT and AST levels testing, distinct changes in myocardial enzymes, coagulation function, and inflammation are well correlated with the clinical data and sequencing data, suggesting that clinical practitioners should monitor the above indicators to track disease progression and guide personalized treatment. In this study, the transcript changes and RNA modification may lend a fresh perspective to our understanding of the SFTSV and play a significant role in the discovery of drugs for effective treatment of this disease.
严重发热伴血小板减少综合征（SFTS）是一种由新型 SFTS 病毒（SFTSV）引起的新型蜱传传染病。在这里，进行了一项纵向采样研究，以探讨 SFTSV 感染后转录水平的差异，并描述住院患者的转录组和表观遗传特征。结果显示，从发病到恢复，某些基因的 mRNA 表达水平发生了显著变化。此外，mA-seq 揭示某些与免疫调节相关的基因可能受到 mA 的调节。除了血小板计数、血清 ALT 和 AST 水平等常规检测外，心肌酶、凝血功能和炎症的明显变化与临床数据和测序数据密切相关，这表明临床医生应监测上述指标，以跟踪疾病进展并指导个性化治疗。在这项研究中，转录变化和 RNA 修饰可能为我们理解 SFTSV 病毒提供新的视角，并在发现有效治疗该疾病的药物方面发挥重要作用。
