Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Department of Dermatology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Methods Mol Biol. 2022;2374:59-71. doi: 10.1007/978-1-0716-1701-4_6.
The identity of ventral neural progenitors in the neural tube is largely dependent on Hedgehog (Hh) signaling. Variations in staining patterns are excellent indicators of aberrant Hh signaling. Here we describe the basic protocol to stain for progenitor populations based on transcription factor expression. We also provide an overview of ciliary and centrosomal staining in the neural tube.
神经管中腹侧神经祖细胞的身份在很大程度上依赖于 Hedgehog（Hh）信号。染色模式的变化是异常 Hh 信号的极好指标。在这里，我们描述了基于转录因子表达来染色祖细胞群体的基本方案。我们还概述了神经管中纤毛和中心体的染色。
