Karastogianni Sophia, Diamantidou Dimitra, Girousi Stella
Analytical Chemistry Laboratory, Chemistry Department, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Biosensors (Basel). 2021 Aug 25;11(9):294. doi: 10.3390/bios11090294.
Voltammetric techniques have been considered as an important analytical tool applied to the determination of trace concentrations of many biological molecules including ascorbic acid. In this paper, ascorbic acid was detected by square wave voltammetry, using graphene oxide paste as a working electrode, modified by a film of a manganese(II) complex compound. Various factors, such as the effect of pH, affecting the response characteristics of the modified electrode were investigated. The relationship between the peak height and ascorbic acid concentration within the modified working electrode was investigated, using the calibration graph. The equation of the calibration graph was found to be: I = 0.0550 + 0.155 with = 0.9998, where I is the SWV current and γac is the mass concentration of ascorbic acid. The LOD and LOQ of the proposed method were determined to be 1.288 μg/L and 3.903 μg/L, respectively. Several compounds, such as riboflavin, biotin, and ions, such as Fe and Cu, were tested and it seemed that they did not interfere with the analytic signal. The proposed procedure was successfully applied in the determination of ascorbic acid in Rosa canina hips.
伏安法技术被认为是一种重要的分析工具，可用于测定包括抗坏血酸在内的许多生物分子的痕量浓度。在本文中，使用氧化石墨烯糊作为工作电极，并通过锰（II）配合物薄膜进行修饰，采用方波伏安法检测抗坏血酸。研究了各种因素，如pH的影响，对修饰电极响应特性的影响。使用校准曲线研究了修饰工作电极内峰高与抗坏血酸浓度之间的关系。校准曲线方程为：I = 0.0550 + 0.155γac，R² = 0.9998，其中I是方波伏安电流，γac是抗坏血酸的质量浓度。该方法的检测限和定量限分别确定为1.288μg/L和3.903μg/L。测试了几种化合物，如核黄素、生物素，以及离子，如Fe和Cu，似乎它们不干扰分析信号。所提出的方法成功应用于犬蔷薇果中抗坏血酸的测定。
