Wei Duncan, Chen Jian, Chen Xiaopu, Wu Shaoyan, Chen Zhaolin, Huang Yinting, Shen Zibin, He Wenzhen
Department of Pharmacy, The First Affiliated Hospital of Shantou University Medical College, No. 57, Changping Road, Guangdong, 515041, Shantou, China.
Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, China.
BMC Neurol. 2021 Sep 25;21(1):370. doi: 10.1186/s12883-021-02397-0.
Ischemic stroke is a disease with high rate of death and disability worldwide. CircRNAs, as a novel type of non-coding RNAs, lacking 5' caps and 3' poly-A tails, has been associated with ischemic stroke. This study aimed to investigate key circRNAs related to ischemic stroke.
RNA sequencing was performed obtain the circRNA expression profiles from peripheral whole blood of three ischemic stroke patients and three healthy individuals. Through bioinformatic analysis, differentially expressed circRNAs (DEcircRNAs) were identified, and GO and pathway analyses for the host genes of DEcircRNAs were conducted. The expression levels of selected circRNAs were analyzed with qRT-PCR. To further explore the functions of key circRNAs, a DEcircRNA-miRNA interaction network was constructed.
A total of 736 DEcircRNAs were detected in ischemic stroke. Functional annotation of host genes of DEcircRNAs revealed several significantly enriched pathways, including Fc epsilon RI signaling pathway, B cell receptor signaling pathway, and T cell receptor signaling pathway. The qRT-PCR results were largely in keeping with our RNA-seq data. The ROC curve analyses indicated that hsa_circ_0000745, hsa_circ_0001459, hsa_circ_0003694 and hsa_circ_0007706 with relatively high diagnostic value. A circRNA-miRNA network, including 1544 circRNA-miRNA pairs, 456 circRNAs and 4 miRNAs, was obtained.
The results of our study may help to elucidate the specific mechanism underlying ischemic stroke.
缺血性中风是一种在全球范围内具有高死亡率和高致残率的疾病。环状RNA（circRNAs）作为一种新型的非编码RNA，缺乏5'帽和3'多聚腺苷尾巴，已被证明与缺血性中风有关。本研究旨在探究与缺血性中风相关的关键环状RNA。
对三名缺血性中风患者和三名健康个体的外周全血进行RNA测序以获得环状RNA表达谱。通过生物信息学分析，鉴定出差异表达的环状RNA（DEcircRNAs），并对DEcircRNAs的宿主基因进行基因本体（GO）和通路分析。用定量逆转录聚合酶链反应（qRT-PCR）分析所选环状RNA的表达水平。为进一步探究关键环状RNA的功能，构建了DEcircRNA-微小RNA（miRNA）相互作用网络。
在缺血性中风中总共检测到736个DEcircRNAs。DEcircRNAs宿主基因的功能注释揭示了几个显著富集的通路，包括FcεRI信号通路、B细胞受体信号通路和T细胞受体信号通路。qRT-PCR结果与我们的RNA测序数据基本一致。受试者工作特征（ROC）曲线分析表明，hsa_circ_0000745、hsa_circ_0001459、hsa_circ_0003694和hsa_circ_0007706具有相对较高的诊断价值。获得了一个包含1544对circRNA-miRNA、456个circRNA和4个miRNA的circRNA-miRNA网络。
我们的研究结果可能有助于阐明缺血性中风的具体潜在机制。
