Kumar Krishan, Yadav Nitin, Banerjee Jyotirmoy, Tripathi Manjari, Sharma M C, Lalwani Sanjeev, Siraj Fouzia, Chandra P Sarat, Sengupta Shantanu, Dixit Aparna Banerjee
Dr. B.R. Ambedkar Center for Biomedical Research (ACBR), University of Delhi, New Delhi, India; Center of Excellence for Epilepsy, AIIMS, New Delhi, India.
Center of Excellence for Epilepsy, AIIMS, New Delhi, India; Department of Biophysics, AIIMS, New Delhi, India.
Epilepsy Res. 2021 Nov;177:106773. doi: 10.1016/j.eplepsyres.2021.106773. Epub 2021 Sep 22.
Focal cortical dysplasia (FCD) is a common pathology responsible for drug-resistant epilepsy (DRE). Failure to precisely localize the epileptogenic zones (EZs) is a major reason for poor surgical outcome in FCD. Currently, there are no molecular or cellular biomarkers available which can aid in defining the EZs in FCD. Phospholipid alterations between healthy and malignant tumor tissues are reported and have been used for marking tumor margins. In this study, we utilize liquid chromatography and tandem mass spectrometry to identify altered lipids in resected brain specimens from FCD patients compared to non-epileptic controls. Based on these results, we propose that a similar approach utilizing unique lipid mass spectra can be used for defining the EZs in FCD. The observed distinct lipid mass spectra of cortical tissues from FCD patients could be used for real-time guidance during surgery as well as for ex vivo examination of resected tissues for diagnostic purposes.
局灶性皮质发育不良（FCD）是导致药物难治性癫痫（DRE）的常见病理原因。未能精确确定致痫区（EZs）是FCD手术效果不佳的主要原因。目前，尚无可用的分子或细胞生物标志物来帮助确定FCD中的EZs。据报道，健康组织与恶性肿瘤组织之间存在磷脂改变，且已用于标记肿瘤边缘。在本研究中，我们利用液相色谱和串联质谱法，来识别FCD患者切除的脑标本与非癫痫对照相比的脂质变化。基于这些结果，我们提出，利用独特脂质质谱的类似方法可用于确定FCD中的EZs。FCD患者皮质组织中观察到的独特脂质质谱，可用于手术期间的实时引导，以及用于切除组织的体外诊断检查。
