Ogata Shohei, Pereira Juan Antonio Cristian, Jhonny Loza Vega Ariel, Carolina Herbas Perez Gladys, Matsuno Keita, Orba Yasuko, Sawa Hirofumi, Kawamori Fumihiko, Nonaka Nariaki, Nakao Ryo
Laboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Graduate School of Infectious Diseases, Hokkaido University, N 18 W 9, Kita-ku, Sapporo 060-0818, Japan.
Facultad de Ciencias Veterinarias, Universidad Autónoma Gabriel René Moreno, Av. 26 de Febrero Entre Av. Busch y Av. Centenario, Ciudad Universitaria, Modulo 228, Santa Cruz de la Sierra, Bolivia.
Vet Sci. 2021 Sep 7;8(9):188. doi: 10.3390/vetsci8090188.
Latin American countries produce more than a quarter of the world's beef and are a major global supplier of livestock protein. Tick-borne diseases (TBDs) are a major constraint to the livestock industry worldwide, including in Latin America. The aim of this study was to detect and characterise tick-borne pathogens in cattle from Santa Cruz, Bolivia, where no detailed epidemiological data are available. Blood samples were collected from 104 cattle. Apicomplexan parasites were detected by nested PCR amplification of the 18S ribosomal RNA gene (rDNA), and was screened by the PCR amplification of 16S rDNA, followed by characterisation based on the heat shock protein and citrate synthase gene sequences. infection was observed in nine cattle (one and eight ), while infection was detected in thirty-two cattle. A sequencing analysis confirmed the presence of and -like. These results provide the first molecular evidence for the four above-mentioned tick-borne pathogens in cattle in Bolivia. This information improves our understanding of the epidemiology of TBDs and will help in formulating appropriate and improved pathogen control strategies.
拉丁美洲国家的牛肉产量占世界总产量的四分之一以上，是全球主要的牲畜蛋白供应地。蜱传疾病（TBDs）是包括拉丁美洲在内的全球畜牧业的主要制约因素。本研究的目的是在玻利维亚圣克鲁斯的牛群中检测并鉴定蜱传病原体，该地区尚无详细的流行病学数据。从104头牛采集了血样。通过对18S核糖体RNA基因（rDNA）进行巢式PCR扩增检测顶复门寄生虫，并通过对16S rDNA进行PCR扩增进行筛查，随后根据热休克蛋白和柠檬酸合酶基因序列进行鉴定。在九头牛（一头环形泰勒虫和八头巴贝斯虫）中观察到感染，而在三十二头牛中检测到无浆体感染。测序分析证实存在环形泰勒虫和巴贝斯虫样。这些结果为玻利维亚牛群中上述四种蜱传病原体提供了首个分子证据。这些信息增进了我们对蜱传疾病流行病学的了解，并将有助于制定适当且改进的病原体控制策略。
