Department of Applied Biology and Chemical Technology, Faculty of Applied Science and Textiles, The Hong Kong Polytechnic University, Hung Hom, Hong Kong.
Research Institute for Future Food, The Hong Kong Polytechnic University, Hung Hom, Hong Kong.
Toxins (Basel). 2021 Sep 6;13(9):624. doi: 10.3390/toxins13090624.
As a sequel to our previous report of the existence of species-specific protein/peptide expression profiles (PEPs) acquired by mass spectrometry in some dinoflagellates, we established, with the help of a plasma-membrane-impermeable labeling agent, a surface amphiesmal protein extraction method (SAPE) to label and capture species-specific surface proteins (SSSPs) as well as saxitoxins-producing-species-specific surface proteins (Stx-SSPs) that face the extracellular space (i.e., SSSPs and Stx-SSPs). Five selected toxic dinoflagellates, , and were used in this study. Transcriptomic databases of these five species were also constructed. With the aid of liquid chromatography linked-tandem mass spectrometry (LC-MS/MS) and the transcriptomic databases of these species, extracellularly facing membrane proteomes of the five different species were identified. Within these proteomes, 16 extracellular-facing and functionally significant transport proteins were found. Furthermore, 10 SSSPs and 6 Stx-SSPs were identified as amphiesmal proteins but not facing outward to the extracellular environment. We also found SSSPs and Stx-SSPs in the proteomes. The potential functional correlation of these proteins towards the production of saxitoxins in dinoflagellates and the degree of species specificity were discussed accordingly.
作为我们之前关于通过质谱法在一些甲藻中获得物种特异性蛋白/肽表达谱 (PEP) 的存在的报告的续集，我们在一种不能渗透质膜的标记试剂的帮助下，建立了一种表面脂筏蛋白提取方法 (SAPE)，以标记和捕获面向细胞外空间的物种特异性表面蛋白 (SSSPs) 和产石房蛤毒素的物种特异性表面蛋白 (Stx-SSPs)（即 SSSPs 和 Stx-SSPs）。本研究使用了五种选定的有毒甲藻、、、和。还构建了这五个物种的转录组数据库。借助液相色谱串联质谱 (LC-MS/MS) 和这些物种的转录组数据库，鉴定了这五个不同物种的细胞外膜蛋白组。在这些蛋白质组中，发现了 16 种细胞外膜的转运蛋白，具有重要的功能。此外，还鉴定出 10 种 SSSP 和 6 种 Stx-SSP 为脂筏蛋白，但不面向细胞外环境。我们还在蛋白质组中发现了 SSSP 和 Stx-SSP。讨论了这些蛋白在甲藻中产石房蛤毒素的潜在功能相关性及其物种特异性程度。
