miR-363-3p/BTG2 在膀胱癌转移中的生物学功能。

Biological functions of miR-363-3p/BTG2 in the metastasis of bladder cancer.

机构信息

Department of Urologic Surgery, Yantaishan Hospital, Yantai, China.

出版信息

J BUON. 2021 Jul-Aug;26(4):1498-1503.

Abstract

PURPOSE

This study aimed to detect the differential expression of microRNA-363-3p (miR-363-3p) in bladder cancer (BC) samples and to explore its influence on metastasis of BC cells.

METHODS

Expression level of miR-363-3p in 70 cases of BC tissues and paracancerous tissues was detected. After establishing miR-363-3p overexpression model in 253j and RT4 cells, their migratory ability was assessed by Transwell and wound healing assay. The interaction between miR-363-3p and its downstream gene was predicted online and further confirmed by luciferase assay. Their involvement in regulating metastasis of BC cells was finally explored.

RESULTS

MiR-363-3p was downregulated in BC tissues compared with that in the paracancerous tissues. Overexpression of miR-363-3p markedly weakened migratory ability in BC cells. BTG2 was the downstream gene binding miR-363-3p. In addition, overexpression of BTG2 reversed the inhibitory effect of miR-363-3p on BC cell migration.

CONCLUSIONS

MiR-363-3p is lowly expressed in BC samples. It weakens in vitro migratory ability in BC cells through downregulating BTG2.

摘要

目的

本研究旨在检测膀胱癌（BC）样本中 microRNA-363-3p（miR-363-3p）的差异表达，并探讨其对 BC 细胞转移的影响。

方法

检测 70 例 BC 组织和癌旁组织中 miR-363-3p 的表达水平。在 253j 和 RT4 细胞中建立 miR-363-3p 过表达模型后，通过 Transwell 和划痕愈合实验评估其迁移能力。在线预测 miR-363-3p 与其下游基因的相互作用，并通过荧光素酶实验进一步验证。最后探讨它们在调节 BC 细胞转移中的作用。