采用体素内不相干运动扩散加权成像（IVIM）磁共振评估链脲佐菌素诱导糖尿病大鼠早期肾损伤的无创性研究。

Non-invasive investigation of early kidney damage in streptozotocin-induced diabetic rats by intravoxel incoherent motion diffusion-weighted (IVIM) MRI.

机构信息

Medical Imaging Center, Jinan University First Affiliated Hospital, No.613 West Huangpu Avenue, Tianhe District, Guangzhou, 510630, Guangdong, China.

Medical Imaging Center, The Eighth Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong, China.

出版信息

BMC Nephrol. 2021 Sep 26;22(1):321. doi: 10.1186/s12882-021-02530-8.

BACKGROUND

The current study investigated the performance of intravoxel incoherent motion diffusion (IVIM) technology in monitoring early renal injury in streptozotocin rats.

METHODS

Forty-eight Sprague-Dawley (SD) rats were divided into a control group and a diabetic mellitus (DM) group. Six rats in each group were randomly selected for MR scans at four different time points (0, 4, 8, and 12 weeks). The IVIM-derived parameters (D, D*, f and ADC values) of the renal cortex (CO), outer and inner stripe of the outer medulla (OS, IS), and internal medulla (IM) were acquired. Changes in each IVIM-derived parameter over time were analyzed, and differences between the two groups at each point were assessed. The associations between the IVIM parameters and IV collagen expression, urine volume (UV), blood urea nitrogen (BUN), and serum creatinine (Scr) were investigated.

RESULTS

The D and D* values of CO and the ADC values of CO, OS, IS and IM displayed significantly different trends between the two groups over time (P<0.05). In addition, significant correlations were discovered between the D* value of CO and UV and BUN (r=0.527, P=0.033; r=0.617, P=0.005), between the ADC value of IM and BUN (r=0.557, P=0.019) and between the f value of IM and BUN (r=0.527, P=0.033). No correlation was found between IVIM parameters and IV collagen expression and Scr.

CONCLUSIONS

IVIM is a potential sensitive and noninvasive technology for the simultaneous assessment of early renal cortical and medullary injuries induced by diabetes.

背景

本研究旨在探讨体素内不相干运动扩散（IVIM）技术在监测链脲佐菌素诱导的大鼠早期肾损伤中的应用价值。

方法

48 只 Sprague-Dawley（SD）大鼠分为对照组和糖尿病（DM）组。每组各有 6 只大鼠随机在 4 个不同时间点（0、4、8 和 12 周）进行 MRI 扫描。获取肾皮质（CO）、外髓外层（OS）、内髓外层（IS）和内髓（IM）的 IVIM 衍生参数（D、D*、f 和 ADC 值）。分析各 IVIM 衍生参数随时间的变化，并评估各时间点两组间的差异。研究 IVIM 参数与 IV 型胶原表达、尿量（UV）、血尿素氮（BUN）和血清肌酐（Scr）之间的相关性。

结果

CO 的 D 和 D值以及 CO、OS、IS 和 IM 的 ADC 值在两组间随时间的变化趋势存在显著差异（P<0.05）。此外，CO 的 D值与 UV 和 BUN（r=0.527，P=0.033；r=0.617，P=0.005）、IM 的 ADC 值与 BUN（r=0.557，P=0.019）和 IM 的 f 值与 BUN（r=0.527，P=0.033）之间存在显著相关性。IVIM 参数与 IV 型胶原表达和 Scr 之间无相关性。