Burnett Robert W
Clinical Chemistry Laboratory, Hartford Hospital, Hartford, Connecticut 06115.
J Res Natl Bur Stand A Phys Chem. 1972 Sep-Oct;76A(5):483-489. doi: 10.6028/jres.076A.042.
The key to accurate measurement of molar absorptivities is a thorough understanding of the sources of error which appear throughout the measurement procedure. Sources of determinant error will be listed with comments on estimating their magnitude and eliminating them where possible. Sources of random error will be discussed as well as the propagation of both random and determinant errors. There is discussion of the need for accurate values of molar absorptivities using examples from clinical chemistry. Finally, the proper use of accurate absorptivity values in the clinical chemistry laboratory will be considered. Here, emphasis is on the need for a quality assurance system which includes routine checks on such things as wavelength calibration and photometric accuracy of spectrophotometers, calibration of analytical balances, and quality of incoming reagents.
准确测量摩尔吸光系数的关键在于全面理解整个测量过程中出现的误差来源。将列出决定性误差的来源，并对估计其大小以及尽可能消除这些误差进行评论。还将讨论随机误差的来源以及随机误差和决定性误差的传播。将通过临床化学的实例讨论获取准确摩尔吸光系数值的必要性。最后，将考虑在临床化学实验室中正确使用准确的吸光系数值。在此，重点是需要一个质量保证体系，该体系包括对诸如分光光度计的波长校准和光度准确度、分析天平的校准以及进货试剂的质量等事项进行常规检查。
