一种用于测量疏水性溶质水溶性的自动耦合柱液相色谱系统。
An Automated Coupled-Column Liquid Chromatography System for Measuring Aqueous Solubilities of Hydrophobic Solutes.
作者信息
Owens John W, DeVoe Howard, Buckley Thomas J, Wasik Stanley P
机构信息
University of Maryland, College Park, MD 20742.
National Bureau of Standards, Gaithersburg, MD 20899.
出版信息
J Res Natl Bur Stand (1977). 1985 Jan-Feb;90(1):41-48. doi: 10.6028/jres.090.003.
An automated apparatus is described for measuring the aqueous solubility of a sparingly soluble organic compound at many different temperatures. Water is pumped through a generator column packed with a chromatographic support coated with the organic compound, producing a saturated solution. The solute in a measured volume of this solution is extracted with an extractor column and analyzed by high performance liquid chromatography (HPLC). The temperature of the thermostat bath and the operation of the valves and the HPLC are under the control of a microcomputer. Solubility measurements of ethylbenzene obtained with this apparatus have a standard deviation at any one temperature of about 3% of the mean.
描述了一种自动装置，用于在许多不同温度下测量微溶性有机化合物的水溶解度。水被泵入装有涂覆有有机化合物的色谱载体的发生器柱中，产生饱和溶液。用萃取柱萃取该溶液一定体积中的溶质，并通过高效液相色谱法（HPLC）进行分析。恒温浴的温度以及阀门和HPLC的操作由微型计算机控制。用该装置获得的乙苯溶解度测量值在任何一个温度下的标准偏差约为平均值的3%。
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