孤啡肽受体：分布、信号转导及作为 G 蛋白偶联受体的证据不断增加——系统评价。
Nesfatin-1 Receptor: Distribution, Signaling and Increasing Evidence for a G Protein-Coupled Receptor - A Systematic Review.
机构信息
Department of Psychosomatic Medicine and Psychotherapy, University Hospital Tübingen, Tübingen, Germany.
Charité Center for Internal Medicine and Dermatology, Department for Psychosomatic Medicine, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Berlin, Germany.
出版信息
Front Endocrinol (Lausanne). 2021 Sep 10;12:740174. doi: 10.3389/fendo.2021.740174. eCollection 2021.
BACKGROUND
Nesfatin-1 is an 82-amino acid polypeptide, cleaved from the 396-amino acid precursor protein nucleobindin-2 (NUCB2) and discovered in 2006 in the rat hypothalamus. In contrast to the growing body of evidence for the pleiotropic effects of the peptide, the receptor mediating these effects and the exact signaling cascades remain still unknown.
METHODS
This systematic review was conducted using a search in the Embase, PubMed, and Web of Science databases. The keywords "nesfatin-1" combined with "receptor", "signaling", "distribution", "pathway", g- protein coupled receptor", and "binding" were used to identify all relevant articles reporting about potential nesfatin-1 signaling and the assumed mediation a G protein-coupled receptor.
RESULTS
Finally, 1,147 articles were found, of which 1,077 were excluded in several steps of screening, 70 articles were included in this systematic review. Inclusion criteria were studies investigating nesfatin-1's putative receptor or signaling cascade, observational preclinical and clinical studies, experimental studies, registry-based studies, cohort studies, population-based studies, and studies in English language. After screening for eligibility, the studies were assigned to the following subtopics and discussed regarding intracellular signaling of nesfatin-1 including the potential receptor mediating these effects and downstream signaling of the peptide.
CONCLUSION
The present review sheds light on the various effects of nesfatin-1 by influencing several intracellular signaling pathways and downstream cascades, including the peptide's influence on various hormones and their receptors. These data point towards mediation a G protein-coupled receptor. Nonetheless, the identification of the nesfatin-1 receptor will enable us to better investigate the exact mediating mechanisms underlying the different effects of the peptide along with the development of agonists and antagonists.
背景
Nesfatin-1 是一种 82 个氨基酸的多肽，从核结合蛋白 2（NUCB2）的 396 个氨基酸前体蛋白中切割而来，于 2006 年在大鼠下丘脑发现。与肽的多效性作用的证据不断增加形成鲜明对比的是，介导这些作用的受体和确切的信号级联仍然未知。
方法
本系统评价通过在 Embase、PubMed 和 Web of Science 数据库中进行搜索来进行。使用“nesfatin-1”与“受体”、“信号”、“分布”、“途径”、G 蛋白偶联受体”和“结合”相结合的关键词来识别所有报告潜在 nesfatin-1 信号及其假设介导 G 蛋白偶联受体的相关文章。
结果
最终发现了 1147 篇文章，其中在几个筛选步骤中排除了 1077 篇，70 篇文章被纳入本系统评价。纳入标准为研究 nesfatin-1 假定受体或信号级联的文章，观察性临床前和临床研究、实验研究、基于登记的研究、队列研究、基于人群的研究和英语语言研究。在筛选合格后，将这些研究分配到以下子主题，并讨论了 nesfatin-1 的细胞内信号，包括潜在的介导这些作用的受体和肽的下游信号。
结论
本综述阐明了 nesfatin-1 通过影响几种细胞内信号通路和下游级联来发挥各种作用，包括肽对各种激素及其受体的影响。这些数据表明，一种 G 蛋白偶联受体介导了 nesfatin-1 的作用。然而，nesfatin-1 受体的鉴定将使我们能够更好地研究肽的不同作用背后的确切介导机制，以及激动剂和拮抗剂的开发。
Zotero 插件