Kanitthamniyom Pojchanun, Zhou Aiwu, Feng Shilun, Liu Aiqun, Vasoo Shawn, Zhang Yi
Singapore Centre for 3D Printing, School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore, Singapore.
School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore, Singapore.
Microsyst Nanoeng. 2020 Jun 29;6:48. doi: 10.1038/s41378-020-0152-4. eCollection 2020.
Magnetic digital microfluidics (MDM) manipulates fluids in the form of droplets on an open substrate, and incorporates surface energy traps (SETs) to facilitate the droplet manipulation. Conventional MDM devices are fabricated monolithically, which makes it difficult to modify the device configuration without completely overhauling the original design. In this paper, we present a modular MDM architecture that enables rapid on-demand configuration and re-configuration of MDM platforms for customized bioanalyses. Each modular component contains a SET and a Lego-like antistud that fits onto a base board with Lego-like studs. We illustrate the versatility of the modular MDM architecture in biomarker sensing, pathogen identification, antibiotic resistance determination, and biochemical quantification by demonstrating immunoassays, phenotypical assays and enzymatic assays on various modular MDM platforms configured on demand to accomplish the fluidic operations required by assorted bioanalytical assays. The modular MDM architecture promises great potential for point-of-care diagnostics by offering on-demand customization of testing platforms for various categories of diagnostic assays. It also provides a new avenue for microfluidic assay development with its high configurability which would significantly reduce the time and cost of the development cycle.
磁数字微流控（MDM）在开放基板上以液滴形式操控流体，并采用表面能阱（SET）来辅助液滴操控。传统的MDM设备是整体制造的，这使得在不完全 overhaul 原始设计的情况下难以修改设备配置。在本文中，我们提出了一种模块化MDM架构，该架构能够对MDM平台进行快速按需配置和重新配置，以实现定制化生物分析。每个模块化组件都包含一个SET和一个类似乐高反扣的部件，该部件可安装在带有类似乐高扣的基板上。我们通过在按需配置的各种模块化MDM平台上演示免疫测定、表型测定和酶测定，以完成各种生物分析测定所需的流体操作，从而展示了模块化MDM架构在生物标志物传感、病原体鉴定、抗生素抗性测定和生化定量方面的多功能性。模块化MDM架构通过为各类诊断测定提供按需定制的测试平台，有望在即时诊断方面发挥巨大潜力。它还因其高度可配置性为微流控分析开发提供了一条新途径，这将显著减少开发周期的时间和成本。
