Felgner P
Zentralbl Bakteriol Orig A. 1978 Jan;240(1):112-7.
A new quantitative technique is described to perform hererogenous enzyme-linded immunosorbent assay using polysterene sticks as solid phase (Stick-ELISA). Advantage of the technique is a combination of economic antigen and conjugate requirements, rapid and thorough washing procedures, and part adaption to general automation devices present in most hospital laboratories which render the technique equally ideal for small and large series in routine serodiagnosis.
本文描述了一种新的定量技术，该技术使用聚苯乙烯棒作为固相来进行异源酶联免疫吸附测定（棒式酶联免疫吸附测定，Stick-ELISA）。该技术的优点包括所需抗原和结合物成本低、洗涤程序快速且彻底，以及部分适用于大多数医院实验室现有的通用自动化设备，这使得该技术在常规血清学诊断中对于小样本和大样本检测同样理想。
