Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Histología y Embriología, Buenos Aires, Argentina.
Acta Odontol Latinoam. 2021 Aug 1;34(2):91-97. doi: 10.54589/aol.34/2/091.
Although it has been demonstrated that exposure of lactating rats to CrVI delays tooth eruption, the effects of CrVI exposure on bone remodeling in the developing alveolus during tooth eruption remain unknown. Our purpose was to analyze the effect of CrVI in the alveolus of the first lower molar of rats. Thirty-two suckling Wistar rats were divided into two groups. The experimental group received 12.5 mg/kg body weight/day of potassium dichromate dissolved in saline solution by oral gavage as of day 4 of the experiment; the control group received an equal dose of saline solution. Each group was divided into two sub-sets and euthanized at the ages of 9 and 15 days, respectively. Histochemical and histomorphometric studies of the bone surfaces of the developing tooth alveolus were performed. The percentage of bone formation surfaces was lower in experimental animals than in age-matched controls. The percentage of bone resorption surfaces was significantly lower in 9-day-old experimental rats than in controls and significantly higher in 15-day-old experimental rats than in controls. Exposure to CrVI during lactation alters the sequence of bone resorption and formation in the walls of the developing alveolus, both of which are necessary for tooth eruption, thus causing a delay.
尽管已经证明，哺乳期大鼠接触六价铬会延迟牙齿萌出，但六价铬暴露对牙齿萌出过程中发育牙槽骨骨改建的影响尚不清楚。我们的目的是分析六价铬对大鼠下颌第一磨牙牙槽骨的影响。32 只哺乳期 Wistar 大鼠分为两组。实验组从实验第 4 天开始每天通过口服灌胃给予 12.5mg/kg 体重的重铬酸钾溶于生理盐水；对照组给予等量生理盐水。每组再分为两个亚组，分别在 9 日龄和 15 日龄处死。对发育中牙牙槽骨表面进行组织化学和组织形态计量学研究。实验组的成骨表面百分比低于同龄对照组。9 日龄实验组的破骨表面百分比明显低于对照组，15 日龄实验组的破骨表面百分比明显高于对照组。哺乳期接触六价铬会改变发育中牙槽骨壁的骨吸收和形成顺序，这两者都是牙齿萌出所必需的，因此会导致延迟。
