Unidad Ejecutora Lillo (Consejo Nacional de Investigaciones Científicas y Técnicas - Fundación Miguel Lillo), Miguel Lillo 251, S. M. de Tucumán, 4000, Argentina.
Facultad de Ciencias Naturales e Instituto Miguel Lillo (Universidad Nacional de Tucumán), S. M. de Tucumán, 4000, Argentina.
Cladistics. 2021 Oct;37(5):630-638. doi: 10.1111/cla.12459. Epub 2021 May 28.
We recently published a method to infer ancestral landmark-based shape ontogenies that takes into account the possible existence of changes in developmental timing. Here we describe SPASOS, a software to perform that analysis. SPASOS is an open-source Windows program written in C. Input data include landmark coordinates for each specimen -with the corresponding information about developmental timing- and a phylogenetic tree showing the relationships among the species sampled. As output, the program produces image files for an easy visualization of the results and data files useful for post-processing. The program incorporates an interpolating function, based on weighting moving averages, which allows analysis of data with scarce information along the ontogenetic trajectory. An empirical evaluation of this function showed its suitability to fill in incomplete ontogenetic trajectories. Finally, we present the results of a reanalysis in SPASOS of a published dataset, where changes in developmental timing were originally inferred by considering PCA scores as shape variables. Both approaches retrieved the same four largest changes in developmental timing, but differed in the ancestral shapes inferred.
我们最近发表了一种推断基于祖先标志的形状发生的方法，该方法考虑了发育时间变化的可能性。在这里，我们将介绍 SPASOS，一种执行该分析的软件。SPASOS 是一个用 C 编写的开源 Windows 程序。输入数据包括每个标本的地标坐标 - 以及有关发育时间的相应信息 - 以及显示采样物种之间关系的系统发育树。作为输出，该程序生成图像文件，便于直观地显示结果，并生成有用的后处理数据文件。该程序包含一个基于加权移动平均值的插值函数，允许对沿发育轨迹信息量稀少的数据进行分析。对该函数的实证评估表明，它适合于填补不完全的发育轨迹。最后，我们在 SPASOS 中重新分析了一个已发表的数据集，其中发育时间的变化最初是通过将 PCA 分数视为形状变量来推断的。这两种方法都检索到了发育时间变化的前四个最大变化，但推断出的祖先形状不同。
