开发和评估一种琼脂捕获系统 (ACS)，用于通过细菌生长和酶活性测定对不溶性颗粒底物进行高通量筛选。
Development and evaluation of an agar capture system (ACS) for high-throughput screening of insoluble particulate substrates with bacterial growth and enzyme activity assays.
机构信息
Department of Biological Sciences, University of Maryland - Baltimore County, Baltimore, MD, USA.
Department of Biological Sciences, University of Maryland - Baltimore County, Baltimore, MD, USA.
出版信息
J Microbiol Methods. 2021 Nov;190:106337. doi: 10.1016/j.mimet.2021.106337. Epub 2021 Sep 24.
PMID:34571109
Abstract
We describe a method for containing insoluble particulates for use as substrates in either bacterial growth or enzyme assays. This method was designed for high-throughput screening of environmental or engineered bacteria. Benchmarking this method with several model bacteria uncovered phenotypes not observable with the particulate substrates alone.
摘要
我们描述了一种用于容纳不溶性颗粒的方法，这些颗粒可用作细菌生长或酶测定的基质。该方法是专为高通量筛选环境或工程细菌而设计的。用几种模式细菌对该方法进行基准测试，揭示了仅用颗粒基质无法观察到的表型。
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