Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX.
Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX; Department of Radiology, IVR Center, Nara Medical University, Kashihara, Japan.
Nanomedicine. 2022 Jan;39:102465. doi: 10.1016/j.nano.2021.102465. Epub 2021 Sep 24.
Embolic agents used in transarterial embolization for intermediate stage hepatocellular carcinoma reduce blood flow into tumors and can deliver anticancer drugs. Tumor blood supply can be interrupted using doxorubicin-eluting beads (DEB-TACE) or non-loaded beads (TAE) of different calibers. In this preclinical study, we characterized the extent of remaining stressed tumor cells after treatment, hypoxia within the surviving tumor regions, and inflammatory immune cell infiltrates after embolization with 40-60 or 70-150 μm with non-loaded or doxorubicin-loaded beads at 3 and 7 days after treatment. TAE-treated tumors had more stressed and surviving tumor cells after 3 days, irrespective of bead size, compared with DEB-TACE-treated tumors. Hypoxic stress of residual cells increased after treatment with 70-150 μm beads without or with doxorubicin. Treatment with DEB-TACE of 70-150 μm resulted in increased inflammation and proliferation in the adjacent parenchyma. Inflammatory cell infiltrates were reduced at the periphery of tumors treated with 40-60 μm DEB-TACE.
用于中期肝细胞癌经动脉栓塞的栓塞剂可减少肿瘤内的血流，并可输送抗癌药物。可以使用不同粒径的载阿霉素微球（DEB-TACE）或非载药微球（TAE）来阻断肿瘤的血液供应。在这项临床前研究中，我们在治疗后，在第 3 天和第 7 天，对 40-60 或 70-150μm 的非载药或载药微球栓塞后，对剩余的受压肿瘤细胞、存活肿瘤区域内的缺氧情况以及炎症免疫细胞浸润的程度进行了特征描述。与 DEB-TACE 治疗的肿瘤相比，无论微球大小如何，TAE 治疗的肿瘤在 3 天后具有更多的受压和存活的肿瘤细胞。70-150μm 微球治疗后，无论是否载有阿霉素，残余细胞的缺氧应激均增加。70-150μm 的 DEB-TACE 治疗会导致相邻实质内的炎症和增殖增加。用 40-60μm 的 DEB-TACE 治疗肿瘤的外周，炎症细胞浸润减少。
