College of wildlife and protected area, Northeast Forestry University, Harbin 150040, Heilongjiang, PR China.
College of wildlife and protected area, Northeast Forestry University, Harbin 150040, Heilongjiang, PR China.
J Inorg Biochem. 2021 Dec;225:111617. doi: 10.1016/j.jinorgbio.2021.111617. Epub 2021 Sep 20.
Arsenic (As) is widely present in the environment in form of arsenite (As) and arsenate (As). Oxidative stress and inflammation are believed to be the dominant mechanisms of As toxicity in vivo and in vitro. The aim of this study was to investigate whether zinc (Zn) alleviates exogenous gill toxicity in carp induced by As and to gain insight into the underlying mechanisms. Exposure of carp to 2.83 mg AsO/L for 30 days reduced superoxide dismutase activity by 4.0%, catalase by 41.0% and glutathione by 19.8%, while the concentration of malondialdehyde was increased by 16.4% compared to the control group, indicating oxidative stress. After the exposure of carp to As the expression of inflammatory markers, such as interleukin-6, interleukin-8, tumor necrosis factor α and inducible nitric oxide synthase in gill tissue were significantly increased. In addition, the phosphorylation of nuclear factor kappa-B (NF-κB) was increased by 225%. 1 mg ZnCl/L can relieve the toxicity of As based on histopathology, antioxidase activity, qRT-PCR and western results. Zn attenuated As-induced gill toxicity that suppressed intracellular oxidative stress and NF-κB pathway by an upregulation of metallothionein. Therefore, the toxic effect of As on the gill cells of carp was reduced. This study provides a theoretical basis for exploring the alleviation of the toxic effects of metalloids on organisms by heavy metals and the biological assessment of the effects.
砷（As）以亚砷酸盐（As）和砷酸盐（As）的形式广泛存在于环境中。氧化应激和炎症被认为是砷在体内和体外毒性的主要机制。本研究旨在探讨锌（Zn）是否能减轻鲤鱼暴露于砷引起的外鳃毒性，并深入了解其潜在机制。鲤鱼暴露于 2.83mg AsO/L 30 天后，超氧化物歧化酶活性降低了 4.0%，过氧化氢酶降低了 41.0%，谷胱甘肽降低了 19.8%，而丙二醛的浓度比对照组增加了 16.4%，表明发生了氧化应激。鲤鱼暴露于砷后，鳃组织中炎症标志物如白细胞介素-6、白细胞介素-8、肿瘤坏死因子-α和诱导型一氧化氮合酶的表达显著增加。此外，核因子 kappa-B（NF-κB）的磷酸化增加了 225%。1mg ZnCl/L 可以基于组织病理学、抗氧化酶活性、qRT-PCR 和 Western 结果缓解砷的毒性。Zn 通过上调金属硫蛋白来减轻 As 诱导的鳃毒性，抑制细胞内氧化应激和 NF-κB 通路。因此，降低了 As 对鲤鱼鳃细胞的毒性。本研究为探索重金属对类金属生物毒性的缓解作用以及生物效应评估提供了理论依据。
