Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China; Ministry of Agriculture Key Laboratory of Tropical Agricultural Environment, South China Agricultural University, Guangzhou 510642, China.
Guangdong AIB Polytechnic.
Ecotoxicol Environ Saf. 2021 Dec 15;226:112827. doi: 10.1016/j.ecoenv.2021.112827. Epub 2021 Sep 24.
Reducing the production of odor during swine breeding has attracted attention. Ammonia (NH) and hydrogen sulfide (HS) contributed to the odor emissions from swine breeding because NH emissions are high and hydrogen sulfide (HS) has a low odor threshold. Sodium butyrate reduces the odor emissions caused by NH and HS, but the corresponding mechanism is unclear. After mixing the feces of six fattening pigs, the mixture was used to process in vitro fermentation experiment. The purpose was researching the effect of sodium butyrate reduced NH and HS emissions in swine cecal contents. The control group was denoted CK, and the treatment groups with different sodium butyrate concentrations (0.015%, 0.030% and 0.150%) were denoted L, M and H. The NH, HS, total gas production and physicochemical indexes were measured, and the bacterial communities in the fermented product were analyzed by 16 S rDNA sequencing. The results showed that group M reduced NH, HS and total gas production by 17.96%, 12.26% and 30.30%, respectively. Sodium butyrate promoted SO accumulation and lowered the pH. Importantly, sodium butyrate decreased the relative abundance of bacteria positively correlated with NH and HS production, but increased the negatively correlated ones. Proteobacteria made a greater contribution to reducing emissions than did other bacterial phyla. Our results showed that adding 0.030% sodium butyrate can significantly reduce NH and HS production, which occurred via alterations in the physicochemical indicators to adjust the abundance of the bacteria related to odor production, including Proteobacteria.
减少养猪过程中的气味产生引起了关注。氨气（NH）和硫化氢（HS）导致了养猪过程中的气味排放，因为 NH 排放量大，而硫化氢（HS）的气味阈值低。丁酸钠可以减少 NH 和 HS 引起的气味排放，但相应的机制尚不清楚。将 6 头育肥猪的粪便混合后，进行体外发酵实验。目的是研究丁酸钠对猪盲肠内容物中 NH 和 HS 排放减少的影响。对照组表示为 CK，不同丁酸钠浓度（0.015%、0.030%和 0.150%）的处理组表示为 L、M 和 H。测量了 NH、HS、总气体产量和理化指标，并通过 16S rDNA 测序分析了发酵产物中的细菌群落。结果表明，M 组使 NH、HS 和总气体产量分别减少了 17.96%、12.26%和 30.30%。丁酸钠促进了 SO 的积累并降低了 pH 值。重要的是，丁酸钠降低了与 NH 和 HS 产生呈正相关的细菌的相对丰度，但增加了与负相关的细菌的相对丰度。变形菌门对减少排放的贡献大于其他细菌门。我们的研究结果表明，添加 0.030%的丁酸钠可以显著减少 NH 和 HS 的产生，这是通过改变理化指标来调节与气味产生相关的细菌丰度实现的，其中包括变形菌门。
