Laury-Kleintop L D, Alhadeff J A, Damjanov I
J Natl Cancer Inst. 1986 Apr;76(4):649-52. doi: 10.1093/jnci/76.4.649.
Isoelectric focusing profiles of alpha-L-fucosidase recovered from human embryonal carcinoma (EC) and seminoma were compared with those of mouse germ cell tumor-derived stem cells and some human somatic cell neoplasms in an attempt to determine whether EC cells could be used as a source of human basic isoelectric forms of the enzyme previously identified in normal and malignant mouse embryonic cells. alpha-L-Fucosidase activity in all human tumors was associated with isoelectric forms in the isoelectric point (pl) range between approximately 4.5 and 7, corresponding to the range seen in normal human tissues. The basic isoelectric forms (pl values, 7.5-9.5) that predominate in the embryonic isoelectric focusing pattern in mouse were not found in human neoplasms. The present data illustrate another difference between human and mouse EC and show that mouse EC cells are not a complete replica of equivalent human tumor cells.
将从人胚胎癌（EC）和精原细胞瘤中回收的α-L-岩藻糖苷酶的等电聚焦图谱与小鼠生殖细胞肿瘤衍生的干细胞和一些人类体细胞肿瘤的图谱进行比较，以确定EC细胞是否可作为先前在正常和恶性小鼠胚胎细胞中鉴定出的该酶的人类碱性等电形式的来源。所有人类肿瘤中的α-L-岩藻糖苷酶活性均与等电点（pl）范围约为4.5至7的等电形式相关，这与在正常人类组织中观察到的范围相对应。在人类肿瘤中未发现小鼠胚胎等电聚焦图谱中占主导的碱性等电形式（pl值为7.5 - 9.5）。目前的数据说明了人类和小鼠EC之间的另一个差异，并表明小鼠EC细胞并非等效人类肿瘤细胞的完全复制品。
