通过体细胞杂种分析将人类α-L-岩藻糖苷酶的基因位点定位于1号染色体。
Assignment of the gene locus for human alpha-L-fucosidase to chromosome 1 by analysis of somatic cell hybrids.
作者信息
Turner B M, Smith M, Turner V S, Kucherlapati R S, Ruddle F H, Hirschhorn K
出版信息
Somatic Cell Genet. 1978 Jan;4(1):45-54. doi: 10.1007/BF01546492.
The alpha-L-fucosidases (EC 3.2.1.51) from human and mouse cells could be separated by isoelectric focusing of neuraminidase-treated cell extracts in acrylamide slab gels. Fourteen hybrid clones derived from the fusion of mouse and human cultured fibroblasts and 37 hybrid clones derived from the fusion of human long-term lymphoid lines with mouse RAG cells were tested for expression of human alpha-L-fucosidase. A strong correlation between the expression of the human enzyme and the presence or absence of human chromosome 1 was found. The presence of human alpha-L-fucosidase in clones scored as positive by isoelectric focusing was confirmed by Ouchterlony double immunodiffusion against IgG from rabbits immunized with purified human alpha-L-fucosidase. It is concluded that the structural gene locus for human alpha-L-fucosidase is located on chromosome 1.
人源和鼠源细胞中的α-L-岩藻糖苷酶（EC 3.2.1.51），可通过在丙烯酰胺平板凝胶中对经神经氨酸酶处理的细胞提取物进行等电聚焦来分离。对源自小鼠和人培养成纤维细胞融合的14个杂交克隆，以及源自人长期淋巴细胞系与小鼠RAG细胞融合的37个杂交克隆进行了人α-L-岩藻糖苷酶表达检测。发现人源酶的表达与人类1号染色体的存在与否之间存在强烈相关性。通过用纯化的人α-L-岩藻糖苷酶免疫的兔IgG进行双向免疫扩散，证实了等电聚焦评分呈阳性的克隆中存在人α-L-岩藻糖苷酶。得出结论，人α-L-岩藻糖苷酶的结构基因位点位于1号染色体上。
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