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gga-miR-2954 通过靶向. 调控鸡胚胎成肌细胞的增殖和分化

Male-Biased gga-miR-2954 Regulates Myoblast Proliferation and Differentiation of Chicken Embryos by Targeting .

机构信息

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

Genes (Basel). 2021 Aug 27;12(9):1325. doi: 10.3390/genes12091325.

DOI:10.3390/genes12091325
PMID:34573307
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8470131/
Abstract

Previous studies have shown that gga-miR-2954 was highly expressed in the gonads and other tissues of male chickens, including muscle tissue. , which has functions in mammalian skeletal muscle development, was predicted to be a target gene of gga-miR-2954. The purpose of this study was to investigate whether gga-miR-2954 plays a role in skeletal muscle development by targeting , and evaluate its function in the sexual dimorphism development of chicken muscle. Here, all the temporal and spatial expression profiles in chicken embryonic muscles showed that gga-miR-2954 is highly expressed in males and mainly localized in cytoplasm. Gga-miR-2954 exhibited upregulated expression of in vitro myoblast differentiation stages. Next, through the overexpression and loss-of-function experiments performed in chicken primary myoblasts, we found that gga-miR-2954 inhibited myoblast proliferation but promoted differentiation. During myogenesis, gga-miR-2954 could suppress the expression of , which promoted myoblast proliferation and inhibited the process of myoblast cell differentiation into multinucleated myotubes. Overall, these findings reveal a novel role of gga-miR-2954 in skeletal muscle development through its function of the myoblast proliferation and differentiation by suppressing the expression of . Moreover, gga-miR-2954 may contribute to the sex difference in chicken muscle development.

摘要

先前的研究表明，gga-miR-2954 在雄性鸡的性腺和其他组织中高度表达，包括肌肉组织。而 作为一种在哺乳动物骨骼肌发育中具有功能的基因，被预测为 gga-miR-2954 的靶基因。本研究旨在通过靶向 来探讨 gga-miR-2954 是否在骨骼肌发育中发挥作用，并评估其在鸡肌肉性二型发育中的功能。在这里，所有在鸡胚胎肌肉中的时空表达谱表明，gga-miR-2954 在雄性鸡中高度表达，主要定位于细胞质中。gga-miR-2954 在体外肌母细胞分化阶段表现出上调表达。接下来，通过在鸡原代肌母细胞中进行的过表达和功能丧失实验，我们发现 gga-miR-2954 抑制肌母细胞增殖但促进分化。在肌发生过程中，gga-miR-2954 可以抑制 的表达，促进肌母细胞增殖并抑制肌母细胞向多核肌管分化的过程。总的来说，这些发现揭示了 gga-miR-2954 通过抑制 的表达，在骨骼肌发育中发挥调节肌母细胞增殖和分化的新作用。此外，gga-miR-2954 可能有助于鸡肌肉发育中的性别差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/5cfe8894a2a0/genes-12-01325-g007a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/d85c462bf680/genes-12-01325-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/05755c530dc0/genes-12-01325-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/a610c97856c9/genes-12-01325-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/4e588b851616/genes-12-01325-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/d48a6c6eab0c/genes-12-01325-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/e2c6831eed89/genes-12-01325-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/5cfe8894a2a0/genes-12-01325-g007a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/d85c462bf680/genes-12-01325-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/05755c530dc0/genes-12-01325-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/a610c97856c9/genes-12-01325-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/4e588b851616/genes-12-01325-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/d48a6c6eab0c/genes-12-01325-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/e2c6831eed89/genes-12-01325-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dea/8470131/5cfe8894a2a0/genes-12-01325-g007a.jpg

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