Vozdova Miluse, Kubickova Svatava, Cernohorska Halina, Fröhlich Jan, Rubes Jiri
Department of Genetics and Reproductive Biotechnologies, Central European Institute of Technology-Veterinary Research Institute, 62100 Brno, Czech Republic.
Animals (Basel). 2021 Sep 6;11(9):2614. doi: 10.3390/ani11092614.
The family Cervidae groups a range of species with an increasing economic significance. Their karyotypes share 35 evolutionary conserved chromosomal segments with cattle (). Recent publication of the annotated red deer () whole genome assembly (CerEla1.0) has provided a basis for advanced genetic studies. In this study, we compared the red deer CerEla1.0 and bovine ARS-UCD1.2 genome assembly and used fluorescence in situ hybridization with bovine BAC probes to verify the homology between bovine and deer chromosomes, determined the centromere-telomere orientation of the CerEla1.0 C-scaffolds and specified positions of the cervid evolutionary chromosome breakpoints. In addition, we revealed several incongruences between the current deer and bovine genome assemblies that were shown to be caused by errors in the CerEla1.0 assembly. Finally, we verified the centromere-to-centromere orientation of evolutionarily fused chromosomes in seven additional deer species, giving a support to previous studies on their chromosome evolution.
鹿科包含一系列具有日益增长经济意义的物种。它们的核型与牛共享35个进化保守的染色体片段（）。最近公布的有注释的马鹿（）全基因组组装（CerEla1.0）为深入的遗传学研究提供了基础。在本研究中，我们比较了马鹿CerEla1.0和牛ARS-UCD1.2基因组组装，并使用牛BAC探针进行荧光原位杂交以验证牛和鹿染色体之间的同源性，确定了CerEla1.0 C-支架的着丝粒-端粒方向以及鹿科进化染色体断点的特定位置。此外，我们揭示了当前鹿和牛基因组组装之间的一些不一致之处，这些不一致被证明是由CerEla1.0组装中的错误引起的。最后，我们验证了另外七个鹿种中进化融合染色体的着丝粒到着丝粒方向，为先前关于它们染色体进化的研究提供了支持。
