Cappai Maria Grazia, Wolf Petra, Liesegang Annette, Biggio Giovanni Paolo, Podda Andrea, Varcasia Antonio, Tamponi Claudia, Berlinguer Fiammetta, Cossu Ignazio, Pinna Walter, Cherchi Raffaele
Dipartimento di Medicina Veterinaria, University of Sassari, 07100 Sassari, Italy.
Institute of Nutrition Physiology and Animal Nutrition, University of Rostock, Justus-von-Liebig-Weg 6b, 18059 Rostock, Germany.
Animals (Basel). 2021 Sep 9;11(9):2646. doi: 10.3390/ani11092646.
A viable tool for the monitoring of the systemic condition of the pregnant jenny may be the determination of serum protein fraction (SPF) levels, including metabolic profiling. Tissue development and composition of the growing fetus requires the mother to provide adequate nutrients to its body parts and organs. In this regard, body fluid distribution and strategic molecule transportation can be screened using SPF electropherograms and analysis of intermediate metabolites. The nutritional and health status of 12 jennies (age: 5-8 years; BW at the start: 135-138 kg; Body Condition Score, BCS [1 to 5 points] = 2.25-2.50; 4th month of gestation) were monitored throughout gestation (approximate gestation period 350-356 d). All animals were pasture-fed and were offered hay . Individual blood samples were collected within the 4th, 7th, and 10th month following conception (ultrasound scanning). Serum biochemistry, in particular, the analysis of 6 fractions of serum proteins was carried out. The significant decrease in circulating albumin in jennies from mid- to late-gestation ( < 0.001) suggests a considerable role of dietary amino acids in the synthesis of protein for fetal tissue formation as well as body fluid distribution and blood pressure control of the jenny in those stages. Moreover, α-globulin decreased significantly in late gestation ( < 0.047), corresponding to major organ development in the terminal fetus and supported by lipid transportation in the bloodstream of the jenny. Similarly, α-globulin decreased in late gestation ( < 0.054) as haptoglobin, an important component for the transport of free circulating hemoglobin, is likely used for fetal synthesis. Mid-gestation, appears to be a crucial moment for adequate dietary nutrient supplementation in order to prevent homeostasis perturbation of jennies, as observed in this trial.
监测妊娠母驴全身状况的一个可行工具可能是测定血清蛋白组分（SPF）水平，包括代谢谱分析。发育中胎儿的组织发育和组成要求母体为其身体各部位和器官提供充足营养。在这方面，可以使用SPF电泳图和中间代谢物分析来筛查体液分布和关键分子的运输情况。在整个妊娠期（约350 - 356天）监测了12头母驴（年龄：5 - 8岁；开始时体重：135 - 138千克；体况评分，BCS[1至5分]=2.25 - 2.50；妊娠第4个月）的营养和健康状况。所有动物均在牧场饲养并提供干草。在受孕后的第4、7和10个月（超声扫描）采集个体血样。进行了血清生化检测，特别是对血清蛋白的6个组分进行了分析。妊娠中期到晚期母驴循环白蛋白的显著下降（<0.001）表明，在胎儿组织形成的蛋白质合成以及这些阶段母驴的体液分布和血压控制中，膳食氨基酸发挥了重要作用。此外，α球蛋白在妊娠晚期显著下降（<0.047），这与末期胎儿主要器官的发育相对应，并得到母驴血液中脂质运输的支持。同样，α球蛋白在妊娠晚期下降（<0.054），因为作为游离循环血红蛋白运输的重要成分的触珠蛋白可能用于胎儿合成。正如本试验所观察到的，妊娠中期似乎是进行充足膳食营养补充以防止母驴体内稳态紊乱的关键时期。
