Sekine Tetsuya, Nagai Hiroshi, Hamada-Sato Naoko
Course of Safety Management in Food Supply Chain, Tokyo University of Marine Science and Technology, Konan-4, Minato-ku, Tokyo 108-8477, Japan.
Department of Ocean Sciences, Tokyo University of Marine Science and Technology, Konan-4, Minato-ku, Tokyo 108-8477, Japan.
Foods. 2021 Aug 31;10(9):2048. doi: 10.3390/foods10092048.
Hidakakombu (), commonly known as kelp, is an edible macroalgae mainly grown in the Hidaka region of Hokkaido. Hidakakombu is graded based on its shape and color. Low-grade Hidakakombu has low value and is distributed at a low price. It is desired to establish a method to add value to low-grade Hidakakombu. In this study, low-grade Hidakakombu was fermented by 001 to add value. Fermentation of Hidakaombu enhanced the inhibition of blood pressure elevation due to ACE inhibition. 001 in fermented Hidakakombu remained viable in simulated gastric and intestinal juices. The ACE inhibitory component in 001-fermented Hidakakombu was isolated from the fraction below 3 kDa using high-performance liquid chromatography. The purified amino acid was identified as D-Trp using nuclear magnetic resonance, mass spectroscopy, and optical rotation measurements. This is the first report on the ACE inhibitory activity of D-Trp in 001-fermented Hidakakombu. Hidakakombu fermented by 001 was shown to be a source of probiotics and functional components against hypertension. Therefore, fermentation by 001 was found to be an effective means of adding high value to low-grade Hidakombu.
日高昆布（），通常称为海带，是一种主要生长在北海道日高地区的可食用大型藻类。日高昆布根据其形状和颜色分级。低等级的日高昆布价值低，以低价销售。期望建立一种提高低等级日高昆布价值的方法。在本研究中，低等级日高昆布通过001进行发酵以增加价值。日高昆布的发酵增强了由于ACE抑制而对血压升高的抑制作用。发酵后的日高昆布中的001在模拟胃液和肠液中仍保持活力。使用高效液相色谱法从低于3 kDa的级分中分离出001发酵日高昆布中的ACE抑制成分。通过核磁共振、质谱和旋光测量将纯化的氨基酸鉴定为D-色氨酸。这是关于D-色氨酸在001发酵日高昆布中的ACE抑制活性的首次报道。经001发酵的日高昆布被证明是益生菌和抗高血压功能成分的来源。因此，发现通过001发酵是提高低等级日高昆布高价值的有效手段。
