Antihypertensive and Probiotic Effects of Hidakakombu () Fermented by 001.

Hidakakombu (), commonly known as kelp, is an edible macroalgae mainly grown in the Hidaka region of Hokkaido. Hidakakombu is graded based on its shape and color. Low-grade Hidakakombu has low value and is distributed at a low price. It is desired to establish a method to add value to low-grade Hidakakombu. In this study, low-grade Hidakakombu was fermented by 001 to add value. Fermentation of Hidakaombu enhanced the inhibition of blood pressure elevation due to ACE inhibition. 001 in fermented Hidakakombu remained viable in simulated gastric and intestinal juices. The ACE inhibitory component in 001-fermented Hidakakombu was isolated from the fraction below 3 kDa using high-performance liquid chromatography. The purified amino acid was identified as D-Trp using nuclear magnetic resonance, mass spectroscopy, and optical rotation measurements. This is the first report on the ACE inhibitory activity of D-Trp in 001-fermented Hidakakombu. Hidakakombu fermented by 001 was shown to be a source of probiotics and functional components against hypertension. Therefore, fermentation by 001 was found to be an effective means of adding high value to low-grade Hidakombu.