Lafuente María Teresa, Romero Paco, González-Candelas Luis
Department of Food Biotechnology, Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Av. Agustín Escardino 7, 46980 Paterna, Valencia, Spain.
Foods. 2021 Sep 16;10(9):2196. doi: 10.3390/foods10092196.
is the main postharvest pathogen of citrus fruit. Although the inner fruit peel part (albedo) is less resistant than the outer part (flavedo) to , the global mechanisms involved in their different susceptibility remain unknown. Here, we examine transcriptome differences between both tissues at fruit harvest and in their early responses to infection. At harvest, not only was secondary metabolism, involving phenylpropanoids, waxes, and terpenoids, generally induced in flavedo vs. albedo, but also energy metabolism, transcription factors (TFs), and biotic stress-related hormones and proteins too. Flavedo-specific induced responses to infection might be regulated in part by ERF1 TF, and are related to structural plant cell wall reinforcement. Other induced responses may be related to HO, the synthesis of phenylpropanoids, and the stress-related proteins required to maintain basal defense responses against virulent pathogens, whereas represses some hydrolase-encoding genes that play different functions and auxin-responsive genes in this peel tissue. In infected albedo, the repression of transport and signal transduction prevail, as does the induction of not only the processes related to the synthesis of flavonoids, indole glucosinolates, cutin, and oxylipins, but also the specific genes that elicit plant immunity against pathogens.
是柑橘类水果采后的主要病原菌。尽管果实内果皮部分（白皮层）比外果皮部分（黄皮层）对[病原菌名称未给出]的抗性更低，但其不同易感性所涉及的整体机制仍不清楚。在此，我们研究了果实采收时这两种组织之间的转录组差异以及它们对感染的早期反应。采收时，与黄皮层相比，白皮层中不仅一般会诱导涉及苯丙烷类、蜡和萜类的次生代谢，还会诱导能量代谢、转录因子（TFs）以及与生物胁迫相关的激素和蛋白质。黄皮层对感染的特异性诱导反应可能部分受ERF1转录因子调控，并且与植物细胞壁结构强化有关。其他诱导反应可能与HO、苯丙烷类的合成以及维持对致病病原体的基础防御反应所需的胁迫相关蛋白有关，而[病原菌名称未给出]会抑制该果皮组织中一些具有不同功能的水解酶编码基因和生长素响应基因。在受感染的白皮层中，运输和信号转导的抑制占主导，与类黄酮、吲哚硫代葡萄糖苷、角质和氧化脂质合成相关的过程以及引发植物对病原体免疫的特定基因的诱导也是如此。
