College of Food Science, Southwest University, Chongqing 400715, P. R. China.
Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, P. R. China.
J Agric Food Chem. 2021 May 26;69(20):5671-5682. doi: 10.1021/acs.jafc.1c01411. Epub 2021 May 14.
is the primary pathogen that causes serious yield losses worldwide. In our previous study, CsWRKY transcription factors (TFs) and some genes associated with immunity were identified in citrus fruits after infection, but little information is available in the literature on the mechanisms of TFs in citrus disease resistance. In this study, the possible mechanisms of CsWRKY65 participating in the establishment of disease resistance were investigated. Results show that CsWRKY65 was a transcriptional activator in the nucleus. The dual-luciferase transient assays and electrophoretic mobility shift assays showed that CsWRKY65 bound with , , , and promoters to activate gene transcription. Besides, the transient overexpression of induced reactive oxygen species accumulation and increased gene expression in leaves. The transient overexpression of in the citrus peel enhanced the disease resistance against . In conclusion, CsWRKY65 is likely to be involved in regulating the disease resistance to of citrus fruits by directly activating the expressions of , , , and . This study provides new information for the mechanism of citrus WRKY TFs participating in the establishment of disease resistance.
是一种主要病原体，可导致全球范围内严重的产量损失。在我们之前的研究中，在感染柑橘果实后鉴定出 CsWRKY 转录因子（TFs）和一些与免疫相关的基因，但关于 TFs 在柑橘抗病性中的作用机制的文献资料很少。在这项研究中，研究了 CsWRKY65 参与建立抗病性的可能机制。结果表明，CsWRKY65 是核内的转录激活因子。双荧光素酶瞬时测定和电泳迁移率变动分析表明，CsWRKY65 与 、 、 和 启动子结合，激活基因转录。此外，瞬时过表达 在 叶片中诱导活性氧物质积累，并增加 基因表达。在柑橘果皮中瞬时过表达 增强了对 的抗病性。总之，CsWRKY65 可能通过直接激活 、 、 、和 的表达，参与调节柑橘果实对 的抗病性。本研究为柑橘 WRKY TFs 参与抗病性建立的机制提供了新的信息。
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