Mok Kevin, Suratanon Narissara, Roytrakul Sittiruk, Charoenlappanit Sawanya, Patumcharoenpol Preecha, Chatchatee Pantipa, Vongsangnak Wanwipa, Nakphaichit Massalin
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
Specialized Research Unit: Probiotics and Prebiotics for Health, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
J Fungi (Basel). 2021 Sep 13;7(9):748. doi: 10.3390/jof7090748.
Association between the gut mycobiome and atopic dermatitis was investigated in 9-12-month-old infants using metagenomics. Two groups of atopic dermatitis infants were classified according to their symptom development as outgrown (recovered) and persisted (still undergoing). The evenness and diversity of the mycobiome in the persisted group were higher than in the healthy and outgrown groups. Dysbiosis of the microbiome in the persisted group was observed by a reduction in the / ratio. Five fungi were selected as markers from each sample group. In the persisted group, sp. abundance increased significantly, while sp. and sp. abundance increased in the healthy group, and sp. and sp. abundance increased considerably in the outgrown group. Metaproteomic analysis revealed that the persisted group had a high abundance of fungal proteins, particularly those from sp. Unique proteins such as RAN-binding protein 1 and glycerol kinase from sp. were hypothesized to be related to atopic dermatitis manifestation in infants.
利用宏基因组学对9至12个月大婴儿的肠道真菌群落与特应性皮炎之间的关联进行了研究。两组特应性皮炎婴儿根据其症状发展情况分为已痊愈（恢复）组和持续患病（仍在患病）组。持续患病组中真菌群落的均匀度和多样性高于健康组和已痊愈组。通过/比值的降低观察到持续患病组中微生物群落的失调。从每个样本组中选择了5种真菌作为标志物。在持续患病组中， 菌属丰度显著增加，而在健康组中 菌属和 菌属丰度增加，在已痊愈组中 菌属和 菌属丰度大幅增加。元蛋白质组分析显示，持续患病组中真菌蛋白丰度较高，尤其是来自 菌属的真菌蛋白。推测来自 菌属的独特蛋白如RAN结合蛋白1和甘油激酶与婴儿特应性皮炎的表现有关。
