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近红外光谱学概述及其在转基因生物检测中的应用。

An Overview of Near Infrared Spectroscopy and Its Applications in the Detection of Genetically Modified Organisms.

机构信息

Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea.

Institute for Future Environmental Ecology Co., Ltd., Jeonju 54883, Korea.

出版信息

Int J Mol Sci. 2021 Sep 14;22(18):9940. doi: 10.3390/ijms22189940.

Abstract

Near-infrared spectroscopy (NIRS) has become a more popular approach for quantitative and qualitative analysis of feeds, foods and medicine in conjunction with an arsenal of chemometric tools. This was the foundation for the increased importance of NIRS in other fields, like genetics and transgenic monitoring. A considerable number of studies have utilized NIRS for the effective identification and discrimination of plants and foods, especially for the identification of genetically modified crops. Few previous reviews have elaborated on the applications of NIRS in agriculture and food, but there is no comprehensive review that compares the use of NIRS in the detection of genetically modified organisms (GMOs). This is particularly important because, in comparison to previous technologies such as PCR and ELISA, NIRS offers several advantages, such as speed (eliminating time-consuming procedures), non-destructive/non-invasive analysis, and is inexpensive in terms of cost and maintenance. More importantly, this technique has the potential to measure multiple quality components in GMOs with reliable accuracy. In this review, we brief about the fundamentals and versatile applications of NIRS for the effective identification of GMOs in the agricultural and food systems.

摘要

近红外光谱(NIRS)技术已成为一种更为流行的方法,可结合大量化学计量学工具,对饲料、食品和药品进行定量和定性分析。这也是 NIRS 在遗传学和转基因监测等其他领域变得越来越重要的基础。相当多的研究已经利用 NIRS 对植物和食品进行了有效识别和区分,特别是对转基因作物的识别。之前的一些综述详细阐述了 NIRS 在农业和食品中的应用,但没有全面的综述比较 NIRS 在检测转基因生物(GMOs)方面的应用。这一点尤为重要,因为与 PCR 和 ELISA 等先前技术相比,NIRS 具有一些优势,例如速度(消除耗时的程序)、无损/非侵入性分析,并且在成本和维护方面较为廉价。更重要的是,该技术具有在 GMOs 中可靠准确地测量多个质量成分的潜力。在本综述中,我们简要介绍了 NIRS 的基本原理和多种应用,以有效识别农业和食品系统中的 GMOs。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adbe/8469702/eefc5b1f7e28/ijms-22-09940-g001.jpg

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