Tamura Y
Bull Tokyo Med Dent Univ. 1986 Mar;33(1):9-22.
Contact microradiographic study and histological study were performed to investigate the sequential changes in the bone marrow after a large dose of vinblastine or colchicine administration. Rats were injected intravenously with various dose of vinblastine or colchicine. The following dynamic histological changes were noted in the bone marrow in the vinblastine 2.0 mumol/kg and colchicine 2.5 mumol/kg-injected groups: Necrosis of the bone marrow cells was observed in the early stage and the necrotic bone marrow cells were replaced by many fibroblast-like cells. After that, the fibroblast-like cells disappeared and the network-like calcified tissue replaced the region where the fibroblast-like cells existed previously. The osteoblasts were lined on the surface of the ectopic calcified tissue and then the osteoblasts were replaced by the typical osteoclasts. In consequence of this dynamic alteration of the cells, the ectopic calcified tissue decreased and the normal bone marrow cells increased in the bone marrow cavity. The ectopic calcification could hardly be observed in the area where the fibroblast-like cells had not appeared. These findings suggested that the markedly proliferated fibroblast-like cells in the marrow cavity after the administration of vinblastine or colchicine may differentiate to osteoblasts and that the osteoclasts may be derived from the bone marrow cells which had already recovered from the toxic effect of the drugs. The mechanism of the dynamic histological changes in the bone marrow remains to be investigated.
进行了接触式显微放射学研究和组织学研究，以调查大剂量长春碱或秋水仙碱给药后骨髓的一系列变化。给大鼠静脉注射不同剂量的长春碱或秋水仙碱。在注射长春碱2.0 μmol/kg和秋水仙碱2.5 μmol/kg的组中，骨髓出现了以下动态组织学变化：早期观察到骨髓细胞坏死，坏死的骨髓细胞被许多成纤维细胞样细胞取代。之后，成纤维细胞样细胞消失，网状钙化组织取代了先前存在成纤维细胞样细胞的区域。成骨细胞排列在异位钙化组织的表面，随后成骨细胞被典型的破骨细胞取代。由于细胞的这种动态变化，骨髓腔中异位钙化组织减少，正常骨髓细胞增加。在未出现成纤维细胞样细胞的区域几乎观察不到异位钙化。这些发现表明，长春碱或秋水仙碱给药后骨髓腔中显著增殖的成纤维细胞样细胞可能分化为成骨细胞，破骨细胞可能来源于已从药物毒性作用中恢复的骨髓细胞。骨髓动态组织学变化的机制仍有待研究。
