Imagawa Naoko, Inoue Kazuya, Matsumoto Keisuke, Omori Michi, Yamamoto Kayoko, Nakajima Yoichiro, Kato-Kogoe Nahoko, Nakano Hiroyuki, Thi Minh Le Phuc, Yamaguchi Seiji, Ueno Takaaki
Department of Dentistry and Oral Surgery, Division of Medicine for Function and Morphology of Sensor Organs, Faculty of Medicine, Osaka Medical and Pharmaceutical University, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan.
Department of Biomedical Science, College of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan.
Materials (Basel). 2021 Sep 17;14(18):5360. doi: 10.3390/ma14185360.
Jaw reconstruction using an additive-manufacturing titanium artificial bone (AMTAB) has recently attracted considerable attention. The synthesis of a titanium artificial bone is based on three-dimensional computed tomography images acquired before surgery. A histological evaluation of porous AMTAB (pAMTAB) embedded in rat calvarial bone defects was conducted. This study examined three groups: rats implanted with mixed-acid and heat-treated pAMTAB, rats implanted with untreated pAMTAB, and rats with no implant. In both pAMTAB groups, bone defects were created in rat calvarial bones using a 5-mm trephine bar, followed by pAMTAB implantation. The pAMTAB was fixed to the defect using the fitting force of the surrounding bones. The rats were sacrificed at 4, 8, and 16 weeks after implantation, and the skull was dissected. Undecalcified ground slides were prepared and stained with Villanueva Goldner. Compared with the no implant control group, both pAMTAB groups exhibited new bone formation inside the defect, with greater bone formation in the mixed-acid and heat-treated pAMTAB group than in the untreated pAMTAB group, but the difference was not significant. These data suggest that pAMTAB induces bone formation after implantation in bone defects. Bone formation appears to be enhanced by prior mixed-acid and heat-treated pAMTAB.
使用增材制造钛人工骨（AMTAB）进行颌骨重建最近引起了广泛关注。钛人工骨的合成基于手术前获取的三维计算机断层扫描图像。对嵌入大鼠颅骨缺损的多孔AMTAB（pAMTAB）进行了组织学评估。本研究检查了三组：植入混合酸和热处理pAMTAB的大鼠、植入未处理pAMTAB的大鼠以及未植入的大鼠。在两个pAMTAB组中，使用5毫米环钻在大鼠颅骨上制造骨缺损，然后植入pAMTAB。通过周围骨骼的拟合力将pAMTAB固定在缺损处。在植入后4周、8周和16周处死大鼠，并解剖颅骨。制备未脱钙的磨片并用Villanueva Goldner染色。与未植入对照组相比，两个pAMTAB组在缺损内均表现出新骨形成，混合酸和热处理pAMTAB组的骨形成比未处理pAMTAB组更多，但差异不显著。这些数据表明，pAMTAB在植入骨缺损后可诱导骨形成。预先进行混合酸和热处理的pAMTAB似乎可增强骨形成。
