Szotkowski Martin, Holub Jiří, Šimanský Samuel, Hubačová Klára, Hladká Dagmar, Němcová Andrea, Marová Ivana
Faculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech Republic.
Microorganisms. 2021 Aug 31;9(9):1848. doi: 10.3390/microorganisms9091848.
One of the most addressed topics today is the transfer from a linear model of economics to a model of circular economics. It is a discipline that seeks to eliminate waste produced by various industries. The food industry generates huge amounts of waste worldwide, particularly the coffee industry, and related industries produce millions of tons of waste a year. These wastes have potential utility in biotechnology, and in the production of energy, fuels, fertilizers and nutrients, using green techniques such as anaerobic digestion, co-digestion, composting, enzymatic action, and ultrasonic and hydrothermal carbonization. This work is focused on the biotechnological use of processed spent coffee grounds (SCG) and waste fat/oil materials by some sp. carotenogenic yeasts in the model of circular economics. The results show that selected yeast strains are able to grow on SCG hydrolysate and are resistant to antimicrobial compounds present in media. The most productive strain CCY19-9-6 was chosen for bioreactor cultivation in media with a mixture of coffee lignocellulose fraction and some fat wastes. CCY19-9-6 was able to produce more than 22 g/L of biomass in mixture of SCG hydrolysate and both coffee oil and frying oil. The combined waste substrates induced the production of lipidic metabolites, whereby the production of carotenoids exceeded 5 mg/g of dry biomass. On media with coffee oil, this strain produced high amounts of ubiquinone (8.265 ± 1.648 mg/g) and ergosterol (13.485 ± 1.275 mg/g). Overall, the results prove that a combination of waste substrates is a promising option for the production of carotenoid- and lipid-enriched yeast biomass.
当今最受关注的话题之一是从线性经济模式向循环经济模式的转变。循环经济是一门旨在消除各行业产生的废弃物的学科。食品行业在全球产生了大量废弃物，尤其是咖啡行业，相关产业每年产生数百万吨废弃物。这些废弃物在生物技术以及能源、燃料、肥料和营养物质的生产中具有潜在用途，可采用厌氧消化、共消化、堆肥、酶促作用以及超声和水热碳化等绿色技术。这项工作聚焦于在循环经济模式下，一些产类胡萝卜素酵母对加工后的咖啡渣（SCG）和废油脂材料的生物技术利用。结果表明，所选酵母菌株能够在SCG水解物上生长，并且对培养基中存在的抗菌化合物具有抗性。选择产量最高的菌株CCY19 - 9 - 6在含有咖啡木质纤维素部分和一些脂肪废弃物混合物的培养基中进行生物反应器培养。CCY19 - 9 - 6能够在SCG水解物与咖啡油和煎炸油的混合物中产生超过22 g/L的生物量。混合的废弃底物诱导了脂质代谢产物的产生，其中类胡萝卜素的产量超过5 mg/g干生物量。在含有咖啡油的培养基上，该菌株产生了大量的泛醌（8.265±1.648 mg/g）和麦角固醇（13.485±1.275 mg/g）。总体而言，结果证明废弃底物的组合是生产富含类胡萝卜素和脂质的酵母生物量的一个有前景的选择。
