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揭示酵母中脂质与β-葡聚糖联产的潜力。

Revealing the Potential of Lipid and β-Glucans Coproduction in Yeast.

作者信息

Byrtusová Dana, Shapaval Volha, Holub Jiří, Šimanský Samuel, Rapta Marek, Szotkowski Martin, Kohler Achim, Márová Ivana

机构信息

Faculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech Republic.

Faculty of Science and Technology, Norwegian University of Life Sciences, Postbox 5003, 1432 Ås, Norway.

出版信息

Microorganisms. 2020 Jul 13;8(7):1034. doi: 10.3390/microorganisms8071034.

DOI:10.3390/microorganisms8071034
PMID:32668638
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7409317/
Abstract

Beta (β)-glucans are polysaccharides composed of D-glucose monomers. Nowadays, β-glucans are gaining attention due to their attractive immunomodulatory biological activities, which can be utilized in pharmaceutical or food supplementation industries. Some carotenogenic yeasts, previously explored for lipid and carotenoid coproduction, could potentially coproduce a significant amount of β-glucans. In the present study, we screened eleven for the coproduction of lipids and β-glucans. We examined the effect of four different C/N ratios and eight different osmolarity conditions on the coproduction of lipids and β-glucans. A high-throughput screening approach employing microcultivation in microtiter plates, Fourier Transform Infrared (FTIR) spectroscopy and reference analysis was utilized in the study. Yeast strains CCY 17-18-4 and CCY 20-2-26 were identified as the best coproducers of lipids and β-glucans. In addition, CCY 17-18-4, CCY 20-2-26 and CCY 77-1-1 were identified as the best alternative producers of β-glucans. Increased C/N ratio led to increased biomass, lipid and β-glucans production for several yeast strains. Increased osmolarity had a negative effect on biomass and lipid production while the β-glucan production was positively affected.

摘要

β-葡聚糖是由D-葡萄糖单体组成的多糖。如今，β-葡聚糖因其具有吸引人的免疫调节生物活性而受到关注，这些活性可用于制药或食品补充行业。一些先前被探索用于脂质和类胡萝卜素联产的产类胡萝卜素酵母，有可能大量联产β-葡聚糖。在本研究中，我们筛选了11株用于脂质和β-葡聚糖的联产。我们研究了四种不同的碳氮比和八种不同的渗透压条件对脂质和β-葡聚糖联产的影响。本研究采用了一种高通量筛选方法，即在微量滴定板中进行微培养、傅里叶变换红外（FTIR）光谱分析和参考分析。酵母菌株CCY 17-18-4和CCY 20-2-26被鉴定为脂质和β-葡聚糖最佳联产菌株。此外，CCY 17-18-4、CCY 20-2-26和CCY 77-1-1被鉴定为β-葡聚糖的最佳替代生产菌株。碳氮比的增加导致几种酵母菌株的生物量、脂质和β-葡聚糖产量增加。渗透压的增加对生物量和脂质产量有负面影响，而对β-葡聚糖产量有正面影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/973c/7409317/879713958ed4/microorganisms-08-01034-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/973c/7409317/b5c11fedde19/microorganisms-08-01034-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/973c/7409317/f08099a75a24/microorganisms-08-01034-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/973c/7409317/635a3b6643c8/microorganisms-08-01034-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/973c/7409317/392c95e0a397/microorganisms-08-01034-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/973c/7409317/879713958ed4/microorganisms-08-01034-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/973c/7409317/b5c11fedde19/microorganisms-08-01034-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/973c/7409317/f08099a75a24/microorganisms-08-01034-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/973c/7409317/635a3b6643c8/microorganisms-08-01034-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/973c/7409317/392c95e0a397/microorganisms-08-01034-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/973c/7409317/879713958ed4/microorganisms-08-01034-g005.jpg

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