Liang Jiayuan, Deng Chuanqi, Yu Kefu, Ge Ruiqi, Xu Yongqian, Qin Zhenjun, Chen Biao, Wang Yinghui, Su Hongfei, Huang Xueyong, Huang Wen, Wang Guanghua, Gong Sanqiang
Coral Reef Research Center of China, Guangxi University, Nanning 530004, China.
Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Nanning 530004, China.
Microorganisms. 2021 Sep 16;9(9):1972. doi: 10.3390/microorganisms9091972.
As the problem of ocean warming worsens, the environmental adaptation potential of symbiotic Symbiodiniaceae and bacteria is directly related to the future and fate of corals. This study aimed to analyse the comprehensive community dynamics and physiology of these two groups of organisms in the coral sp. through indoor simulations of heat stress (which involved manually adjusting the temperature between both 26 °C and 34 °C). Heat treatment (≥30 °C) significantly reduced the abundance of Symbiodiniaceae and bacteria by more than 70%. After the temperature was returned to 26 °C for one month, the Symbiodiniaceae density was still low, while the absolute number of bacteria quickly recovered to 55% of that of the control. At this time point, the / value rose to 91% of the pretemperature value. The content of chlorophyll b associated with Cyanobacteria increased by 50% compared with that under the control conditions. Moreover, analysis of the Symbiodiniaceae subclade composition suggested that the relative abundance of C1c.C45, C1, and C1ca increased during heat treatment, indicating that they might constitute heat-resistant subgroups. We suggest that the increase in the absolute number of bacteria during the recovery period could be an important indicator of coral holobiont recovery after heat stress. This study provides insight into the cross-linked regulation of key symbiotic microbes in the coral sp. during high-temperature stress and recovery and provides a scientific basis for exploring the mechanism underlying coral adaptation to global warming.
随着海洋变暖问题的加剧，共生的虫黄藻和细菌的环境适应潜力直接关系到珊瑚的未来和命运。本研究旨在通过室内模拟热应激（手动将温度在26°C和34°C之间调节）来分析珊瑚物种中这两类生物的综合群落动态和生理特征。热处理（≥30°C）使虫黄藻和细菌的丰度显著降低了70%以上。温度恢复到26°C一个月后，虫黄藻密度仍然很低，而细菌的绝对数量迅速恢复到对照的55%。此时，/值升至预处理温度值的91%。与蓝细菌相关的叶绿素b含量比对照条件下增加了50%。此外，对虫黄藻亚分支组成的分析表明，热处理期间C1c.C45、C1和C1ca的相对丰度增加，表明它们可能构成耐热亚群。我们认为恢复期细菌绝对数量的增加可能是热应激后珊瑚共生体恢复的一个重要指标。本研究深入了解了高温胁迫和恢复过程中珊瑚物种关键共生微生物的交联调控，为探索珊瑚适应全球变暖的潜在机制提供了科学依据。
