School of Pharmacy, Jilin Medical University, Jilin 132013, China.
School of Life Sciences, Jilin University, Changchun 130012, China.
Molecules. 2021 Sep 17;26(18):5644. doi: 10.3390/molecules26185644.
Poloxamer188 (PL188), as one of the most commonly used pharmaceutical excipients, has unique physicochemical properties and good biocompatibility, and so is playing an increasingly extensive role in the field of medicine. Currently, there are few studies on the tissue distribution of PL188 in vivo. In this study, the LC-MS method based on MS technique of quadrupole time of flight mass spectrometry for absolute quantitative analysis of poloxamer 188 in biological substrates was established for the first time. The tissue distribution of poloxamer188 in SD rats were studied using the established quantitative analysis method. To explore the distribution of PL188 in organs and tissues, PL188 was administered via rat tail vein at a dose of 5 mg/kg. Eight kinds of tissues including heart, liver, spleen, lung, kidney, stomach, muscle and brain of rats were collected at 0.25 h, 1 h and 4 h after administration. Tissue distributions showed the highest level was observed in kidney, then in stomach, which indicated PL188 mainly bioaccumulated in the kidney. This study can provide references for the further study of PL188.
泊洛沙姆 188（PL188）作为最常用的药用辅料之一，具有独特的物理化学性质和良好的生物相容性，因此在医学领域的应用越来越广泛。目前，关于 PL188 在体内的组织分布的研究较少。本研究首次建立了基于四极杆飞行时间质谱技术的 MS 技术的 LC-MS 方法，用于生物基质中泊洛沙姆 188 的绝对定量分析。使用建立的定量分析方法研究了泊洛沙姆 188 在 SD 大鼠体内的组织分布。为了探索 PL188 在器官和组织中的分布，以 5mg/kg 的剂量经大鼠尾静脉给予 PL188。在给药后 0.25 h、1 h 和 4 h 收集大鼠的心、肝、脾、肺、肾、胃、肌肉和脑等 8 种组织。组织分布显示，在肾脏中观察到最高水平，其次是胃，表明 PL188 主要在肾脏中蓄积。本研究可为 PL188 的进一步研究提供参考。
