School of Pharmacy, Jilin Medical University, Jilin, 132013, PR China.
School of Pharmacy, Yanbian University, Yanji, 133002, PR China.
Anal Methods. 2021 Nov 25;13(45):5516-5522. doi: 10.1039/d1ay01373d.
Poloxamers are commonly used pharmaceutical excipients. They are high molecular weight polymers formed from polypropylene oxide (PPO) and polyethylene oxide (PEO). However, PL124, a low molecular weight example in the poloxamer family, has rarely been reported, and there is no research into its tissue distribution in the body after administration. In this study, rat tissue samples were quantitatively studied UHPLC-Q-TOF/MS after the intravenous administration of 10 mg kg PL124. The quantitative method showed good sensitivity and selectivity. The linear range of PL124 was 0.1-5 μg mL and the LLOQ was 0.1 μg mL. The relative error in terms of the accuracy was no higher than 13.9%, and the relative standard deviation in terms of the precision was no higher than 9.6%. The extraction recovery, matrix effect, and stability results of the established method were also satisfactory. The research showed that PL124 can be quickly distributed to large amounts of tissue, and tissue with higher levels of blood flow has higher concentrations. PL124 could be rapidly eliminated in 4 h from most organs, except the heart and liver. This study can be helpful for the further analysis of PL124.
泊洛沙姆是常用的药用辅料。它们是由聚环氧丙烷（PPO）和聚环氧乙烷（PEO）组成的高分子量聚合物。然而，泊洛沙姆家族中的低分子量实例 PL124 很少被报道，并且在给药后其在体内的组织分布也没有研究。在这项研究中，在静脉注射 10 mg kg PL124 后，使用 UHPLC-Q-TOF/MS 对大鼠组织样品进行定量研究。该定量方法显示出良好的灵敏度和选择性。PL124 的线性范围为 0.1-5 μg mL，LLOQ 为 0.1 μg mL。准确度的相对误差不高于 13.9%，精密度的相对标准偏差不高于 9.6%。所建立方法的提取回收率、基质效应和稳定性结果也令人满意。研究表明，PL124 可以快速分布到大量组织中，血流水平较高的组织具有较高的浓度。除心脏和肝脏外，PL124 可以在 4 小时内从大多数器官中迅速消除。本研究可为进一步分析 PL124 提供帮助。
