Bitencourth Karla, Amorim Marinete, Oliveira Stefan Vilges de, Gazêta Gilberto Salles
Laboratório de Referência Nacional em Vetores das Riquetsioses, Fundação Oswaldo Cruz, Rio de Janeiro 21045-900, RJ, Brazil.
Programa de Pós-Graduação em Biodiversidade e Saúde, Fundação Oswaldo Cruz, Rio de Janeiro 21045-900, RJ, Brazil.
Pathogens. 2021 Sep 6;10(9):1146. doi: 10.3390/pathogens10091146.
Regional differences in tick-borne disease epidemiology may be related to biological variations between vector populations. (Ixodida: Ixodidae), a neotropical tick, is known from several regions in Brazil. However, only in the metropolitan area of São Paulo (SP) state are there studies that establish its role as a vector of a pathogenic rickettsia (). The aim of the study was to analyze the genetic diversity, population dynamics, and rickettsia infection in populations from different spotted fever scenarios in Brazil. Samples were subjected to DNA extraction, amplification and sequencing of 12S rDNA, cytochrome oxidase subunit II and D-loop mitochondrial markers for tick population analyses, and A, A, A, and B genes for rickettsia researches. Of the 7-17 tick haplotypes identified, 5-13 were exclusive to each population and 2-12 for each epidemiological scenario, as well as three haplotypes shared by all populations. populations are expanding, and do not appear to be genetically structured vis-a-vis the different epidemiological scenarios studied. (in SP) and (in Santa Catarina) were identified as infecting . No relationship between tick haplotypes and rickettsia types were observed.
蜱传疾病流行病学的区域差异可能与媒介种群之间的生物学差异有关。巴西新热带蜱（硬蜱科：蜱属）在巴西的几个地区都有发现。然而，只有在圣保罗州的大都市区才有研究确定其作为致病性立克次氏体媒介的作用。本研究的目的是分析巴西不同斑疹热疫区的蜱种群的遗传多样性、种群动态和立克次氏体感染情况。对样本进行DNA提取、12S rDNA、细胞色素氧化酶亚基II和线粒体D环标记的扩增及测序以进行蜱种群分析，并对立克次氏体研究进行A、A、A和B基因检测。在所鉴定出的7至17种蜱单倍型中，每个种群有5至13种是独有的，每种流行病学情景有2至12种，还有三种单倍型为所有种群所共有。蜱种群正在扩张，相对于所研究的不同流行病学情景而言，其似乎没有遗传结构。（在圣保罗州）和（在圣卡塔琳娜州）被确定为感染。未观察到蜱单倍型与立克次氏体类型之间存在关联。
