Bitencourth K, Amorim M, de Oliveira S V, Voloch C M, Gazêta G S
Laboratório de Referência Nacional em Vetores das Riquetsioses, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Programa de Pós-Graduação em Biodiversidade e Saúde, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Med Vet Entomol. 2019 Jun;33(2):256-268. doi: 10.1111/mve.12363. Epub 2019 Feb 11.
Amblyomma ovale (Ixodida: Ixodidae) Koch, 1844 is widely-reported in the neotropical region and is the main vector in the epidemic cycle of Rickettsia parkeri strain Atlantic rainforest, a bioagent of a milder variety of spotted fever (SF). Because species with wide geographical distributions are known to exhibit variations that influence their vectorial capacity, the present study aimed to analyze genetic diversity and rickettsia infection of A. ovale collected during the investigation and surveillance of SF cases in the Cerrado and Atlantic rainforest (ARF) Brazilian biomes. Samples had their DNA extracted, amplified and sequenced for 16S rDNA, 12S rDNA, cytochrome oxidase subunit II and D-loop markers for tick analyses, as well as the gltA, htrA, ompA and ompB genes for rickettsia detection. Between 11 and 33 A. ovale haplotypes were identified, all of them exclusive to areas within individual analyzed biome areas. The A. ovale populations appeared to be structured, with Cluster I restricted to Cerrado + ARF isolated in Caatinga and Cluster II to ARF continuous area. Rickettsia bellii, R. parkeri strain Atlantic rainforest (first report for Goiás state, Cerrado), Rickettsia asemboensis (first record in A. ovale for Brazil) and Rickettsia felis (first detection in this ixodid) were identified. A. ovale clusters were not associated with rickettsia types.
卵形钝缘蜱（硬蜱目：硬蜱科），由科赫于1844年发现，在新热带地区广泛分布，是帕克立克次体大西洋雨林株流行周期的主要传播媒介，该立克次体是一种症状较轻的斑点热生物病原体。由于已知地理分布广泛的物种会表现出影响其传播能力的变异，本研究旨在分析在巴西塞拉多和大西洋雨林生物群落对斑点热病例进行调查和监测期间采集的卵形钝缘蜱的遗传多样性和立克次体感染情况。提取样本的DNA，对16S rDNA、12S rDNA、细胞色素氧化酶亚基II和D-loop标记进行扩增和测序以进行蜱分析，同时对gltA、htrA、ompA和ompB基因进行扩增和测序以检测立克次体。共鉴定出11至33个卵形钝缘蜱单倍型，所有单倍型均为各分析生物群落区域内的特有类型。卵形钝缘蜱种群似乎具有结构，第一类群局限于隔离在卡廷加的塞拉多+大西洋雨林，第二类群局限于大西洋雨林连续区域。鉴定出贝利立克次体、帕克立克次体大西洋雨林株（戈亚斯州塞拉多的首次报告）、阿森博立克次体（巴西卵形钝缘蜱中的首次记录）和猫立克次体（该硬蜱中的首次检测）。卵形钝缘蜱类群与立克次体类型无关。
