Li Mengfei, Li Jie, Wei Jianhe, Paré Paul W
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Plants (Basel). 2021 Sep 16;10(9):1931. doi: 10.3390/plants10091931.
The root of the perennial herb is a widely used source for traditional Chinese medicines. While the plant thrives in cool-moist regions of western China, early bolting and flowering (EBF) for young plants significantly reduces root quality and yield. Approaches to inhibit EBF by changes in physiology during the vernalization process have been investigated; however, the mechanism for activating EBF is still limited. Here, transcript profiles for bolted and unbolted plants (BP and UBP, respectively) were compared by transcriptomic analysis, expression levels of candidate genes were validated by qRT-PCR, and the accumulations of gibberellins (GA, GA, GA, GA and GA) were also monitored by HPLC-MS/MS. A total of over 72,000 unigenes were detected with ca. 2600 differentially expressed genes (DEGs) observed in the BP compared with UBP. While various signaling pathways participate in flower induction, it is genes associated with floral development and the sucrose pathway that are observed to be coordinated in EBF plants, coherently up- and down-regulating flowering genes that activate and inhibit flowering, respectively. The signature transcripts pattern for the developmental pathways that drive flowering provides insight into the molecular signals that activate plant EBF.
这种多年生草本植物的根是传统中药广泛使用的来源。虽然该植物在中国西部凉爽湿润的地区生长旺盛，但幼苗的早期抽薹开花（EBF）会显著降低根的质量和产量。人们已经研究了通过在春化过程中改变生理状态来抑制EBF的方法；然而，激活EBF的机制仍然有限。在这里，通过转录组分析比较了抽薹植株和未抽薹植株（分别为BP和UBP）的转录谱，通过qRT-PCR验证了候选基因的表达水平，并通过HPLC-MS/MS监测了赤霉素（GA1、GA3、GA4、GA7和GA9）的积累。共检测到超过72,000个单基因，与UBP相比，在BP中观察到约2600个差异表达基因（DEG）。虽然各种信号通路参与花的诱导，但在EBF植株中观察到与花发育和蔗糖途径相关的基因是协同的，分别上调和下调激活和抑制开花的开花基因。驱动开花的发育途径的标志性转录本模式为激活植物EBF的分子信号提供了见解。
