The Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Jiangnan University, Wuxi, China.
Department of Basic Medicine, Wuxi Medical School, Jiangnan University, Wuxi, China.
Mater Sci Eng C Mater Biol Appl. 2021 Oct;129:112385. doi: 10.1016/j.msec.2021.112385. Epub 2021 Aug 21.
In this study, Ag nanoparticles were firstly reduced on the surface of lignin nanoparticles (LNP) by direct reaction of silver nitrate without the use of a catalyst. Thermogravimetric analysis, Zeta potential and transmission electron microscopy measurements were performed to give evidence of the effectiveness of the reaction. After that, glutaraldehyde crosslinked PVA hydrogels, were produced by addition of unmodified LNP and Ag loaded LNP (LNP) in presence of cellulose nanocrystals (CNC). Thermal, mechanical, rheological, microstructural and biological anti-oxidative and anti-bacterial properties of the resulted hydrogels were investigated. It was proved that all the three nanofillers were homogeneously dispersed in PVA, and the pore diameter of the hydrogels was in the range of 0.5-2.0 μm. Nevertheless, the hydrogels showed high toughness, long-term and repeatable adhesiveness to a variety of substrates. In particular, composite hydrogels containing LNP nanoparticles showed excellent radical scavenging and antibacterial activities. Consequently, the effects of PVA-2CNC-2LNP on wound healing in mice model of full-thickness skin resection were evaluated by hematoxylin and eosin staining, taking as a reference the PVA-2CNC-2LNP system. The results showed that the wound healing time of PVA-2CNC-2LNP group was faster than that of neat PVA and PVA-2CNC, highlighting the role of LNP in enhancing the contact-active anti-oxidative and antibacterial activities mechanism in composite hydrogels. We expected that PVA hydrogels incorporating LNP could be used as green and efficient biomedical wound dressing materials.
在这项研究中，Ag 纳米颗粒通过硝酸银的直接反应在木质素纳米颗粒（LNP）表面上被首次还原，而无需使用催化剂。热重分析、Zeta 电位和透射电子显微镜测量被用来证明反应的有效性。之后，通过加入未修饰的 LNP 和负载 Ag 的 LNP（LNP）以及纤维素纳米晶体（CNC）来制备戊二醛交联的 PVA 水凝胶。研究了所得水凝胶的热学、力学、流变学、微观结构和生物抗氧化和抗菌性能。结果证明，所有三种纳米填料均均匀分散在 PVA 中，水凝胶的孔径在 0.5-2.0μm 范围内。然而，水凝胶表现出高韧性、长期和可重复的对各种基底的粘附性。特别是，含有 LNP 纳米颗粒的复合水凝胶表现出优异的自由基清除和抗菌活性。因此，通过对全厚度皮肤切除小鼠模型进行苏木精和伊红染色，评估了 PVA-2CNC-2LNP 对伤口愈合的影响，将 PVA-2CNC-2LNP 系统作为参考。结果表明，PVA-2CNC-2LNP 组的伤口愈合时间快于纯 PVA 和 PVA-2CNC，突出了 LNP 在增强复合水凝胶中接触活性抗氧化和抗菌活性机制方面的作用。我们期望将 LNP 掺入 PVA 水凝胶可以用作绿色高效的生物医学伤口敷料材料。
