Institute of Functional and Clinical Anatomy, Friedrich-Alexander University Erlangen-Nürnberg, Universitätsstraße 19, 91054, Erlangen, Bavaria, Germany.
WaveLight GmbH, Erlangen, Bavaria, Germany.
Sci Rep. 2021 Sep 27;11(1):19087. doi: 10.1038/s41598-021-98635-z.
The purpose of this study was to elucidate, under which conditions abattoir-acquired pig eyes are suitable for refractive excimer laser experiments. Porcine eyes from tunnel-scalded (n = 5) and tank-scalded (n = 10) pigs were compared to unscalded eyes (n = 5) and to eyes scalded in the laboratory (n = 5). The corneal epithelium was removed before an excimer laser was used to perform a - 8.0 D photoablation. Corneal thickness was measured by optical coherence topography before and after photoablation. The ablation depth was determined with a contour measuring station, the morphology of the ablated areas was characterized by scanning electron microscopy and white-light profilometry. The scalded eyes showed an increase in corneal swelling which gained statistical significance in tank-scalded eyes showing a wedge-shaped opaque stromal lesion in the nasal corneal quadrant. A measurable deterioration of photoablation was only found in tank-scalded eyes that exhibited the opaque lesion. Ablated area morphology was smooth and regular in the unscalded and tunnel-scalded eyes. The tank-scalded eyes showed conspicuous wrinkles. While unscalded eyes should always be preferred for excimer laser laboratory experiments, the data suggest that the use of tunnel-scalded eyes may also be acceptable and should be chosen over tank-scalded eyes.
本研究旨在阐明在何种条件下，屠宰场获得的猪眼适合用于屈光准分子激光实验。对隧道烫（n=5）和水槽烫（n=10）猪的猪眼与未烫眼（n=5）和实验室烫眼（n=5）进行了比较。在使用准分子激光进行-8.0 D光消融之前，去除角膜上皮。在光消融前后通过光学相干断层扫描测量角膜厚度。使用轮廓测量仪确定消融深度，通过扫描电子显微镜和白光轮廓测量术对消融区域的形态进行特征描述。烫眼表现出角膜肿胀增加，在水槽烫眼中具有统计学意义，在鼻侧角膜象限出现楔形不透明基质病变。仅在表现出不透明病变的水槽烫眼中发现可测量的光消融恶化。未烫眼和隧道烫眼中的消融区域形态光滑且规则。水槽烫眼显示出明显的皱纹。虽然未烫眼应始终优先用于准分子激光实验室实验，但数据表明，隧道烫眼的使用也可能是可以接受的，并且应选择优于水槽烫眼。
