Augenklinik, Klinikum rechts der Isar, Technische Universitaet Muenchen, Munich, Germany.
J Cataract Refract Surg. 2010 Aug;36(8):1385-91. doi: 10.1016/j.jcrs.2010.01.033.
To evaluate the possible side effects on human and porcine cadaver eyes induced by excimer laser ablation with 3 ablation frequencies.
Augenklinik, Klinikum rechts der Isar, Technische Universitaet Muenchen, Munich, Germany.
Central epithelial abrasion was performed on porcine and human donor eyes. Next, the porcine eyes were photoablated (-9.00 diopters) at 1 of 3 frequencies (200 Hz, 500 Hz, or 1000 Hz) using a prototype 1000 Hz excimer laser. The human eyes were ablated at 1000 Hz. The surface of the stroma as well as the structure and ultrastructure of the corneal cells and stroma were examined using light microscopy, transmission electron microscopy, and scanning electron microscopy (SEM). Special attention was given to the detection of potential thermal damage and the evaluation of corneal smoothness.
Histopathologic examination showed very low to almost no differences between the 3 repetition rates. In all cases, SEM showed an equally smooth surface.
The structural and ultrastructural evaluation of corneas treated with ablation frequencies of 200 Hz, 500 Hz, and 1000 Hz showed no specific side effects associated with the high repetition rates. The ablation quality was comparable in the 3 frequency groups. However, the treatment time was significantly less with a high repetition rate, indicating the clinical potential in refractive surgery of excimer lasers with a repetition rate of 1000 Hz.
No author has a financial or proprietary interest in any material or method mentioned. Additional disclosures are found in the footnotes.
评估 3 种激光消融频率对人眼和猪眼尸体的潜在副作用。
德国慕尼黑技术大学附属医院眼科。
对猪眼和人眼供体进行中央上皮刮除。然后，使用原型 1000 Hz 准分子激光对猪眼进行 1 次（-9.00 屈光度）光消融，频率分别为 200 Hz、500 Hz 或 1000 Hz。人眼以 1000 Hz 频率进行消融。采用光镜、透射电镜和扫描电镜（SEM）观察基质表面以及角膜细胞和基质的结构和超微结构。特别注意潜在热损伤的检测和角膜光滑度的评估。
组织病理学检查显示，3 种重复率之间差异非常小或几乎没有。在所有情况下，SEM 显示表面同样光滑。
用 200 Hz、500 Hz 和 1000 Hz 消融频率处理的角膜的结构和超微结构评估未显示与高重复率相关的特定副作用。3 个频率组的消融质量相当。然而，高重复率的治疗时间明显减少，表明 1000 Hz 重复率的准分子激光在屈光手术中有临床潜力。
无作者在提到的任何材料或方法上有财务或所有权利益。其他披露信息见脚注。
